In the plant Arabidopsis thaliana, At5g62990.1 is a gene model for the locus At5g62990.[1]
Also known as EMB1692, this gene model is classified by TAIR as "EMB1692 (EMBRYO DEFECTIVE 1692)". It is located on chr5, region 25295054-25296538 FORWARD.
Physiological function[]
At5g62990.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At5g62990.1/Expression
Subcellular localization[]
At5g62990.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At5g62990.1 has 494 amino-acid residues and a molecular weight Mw = 56.788 kDa.
The protein is relatively neutral; at the physiological pH 7.4, its net charge is 1.33, or 0.003 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At5g62990.1/Knockout
Protein interactions[]
At5g62990.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At5g62990.1 has 1 recognized domains:
- Domain_0059_0401 DUF860 domain (PF05898, IPR008578) Description: Protein of unknown function DUF860, plant_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At5g62990.1
MMMALTQLVP
SSPEKLFCFS
SFFLPSIPLE
IRRSNVTTKT
SSKKLGSLSL
NISCSAHKIV
RSPSLDRHVV
KQNRVRFVQK
LNTLLLSKPK
HYIPIEILYK
CRSYLCIENP
LAILSMIRRY
PTIFELFTTP
TPHLPMNATK
PLSQLCVRLT
SAASSLAMQE
LNLKSEISDK
LATKLQKLLM
LSSHRRLLLS
KLVHIAPDFG
FPPNFRSRLC
NDYPDKFKTV
DTSYGRALEL
VSSDPELANQ
MPSPEVDRGL
IVDRPLKFKR
LNLRRGLNLK
RRHQGFLIKF
RESPDVCPYK
MSSDYLASES
IEAEKRACAV
VREVLGLTVE
KRTLIDHLTH
FRKEFSLPNK
LRDLIVRHPE
LFYVSIKGMR
DSVFLVEAYN
DNGDLLDKDE
RLVIRERLID
LIQEGKRIRR
ERRRKGATLG
DKSVEEKRKD
DETIDDYDSD
LDDEYEDGFE
NLFDSEDLGV
EYHFDEEDDD
DEAGVNGESV
EYWSRKLSSS
GSSSDEANSA
VESW
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.