At5g04990.1

In the plant Arabidopsis thaliana, At5g04990.1 is a gene model for the locus At5g04990.^[1]

This gene model is classified by TAIR as "sad1/unc-84 protein-related". It is located on chr5, region 1471699-1473771 REVERSE.

Physiological function[]

At5g04990.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At5g04990.1/Expression

Subcellular localization[]

At5g04990.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At5g04990.1 has 471 amino-acid residues and a molecular weight Mw = 51.497 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is -3.55, or -0.008 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At5g04990.1/Knockout

Protein interactions[]

At5g04990.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At5g04990.1 has 1 recognized domains:

Domain_0313_0451 Sad1_UNC domain (PF07738, IPR012919) Description: Sad1/UNC-like, C-terminal_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At5g04990.1 MSASTVSITA NTAAATRRTP ILAGEKKSNF DYPQSESLAN GGVGEAGGTS RDLSRGEATL DRSQGQDLGP VTRRSVSAAT GTNTTATQRR TRKVATPKSE KARWKTVVRI FAKQLGALLI IVGLIQLTRK MILKASSPSS PISSYETEMA FSGLESRIAE VDGLVKATTN SMQVQVELLD KKMEREAKVL RQEIERKASA FQSELKKIES RTESLEKSVD EVNAKPWVTK DELERIYEEL KKGNVDDSAF SEISIDELRA YARDIMEKEI EKHAADGLGR VDYALASGGA FVMEHSDPYL VGKGSSWFAT TMRRAHTNAV KMLSPSFGEP GQCFPLKGSE GYVQIRLRGP IIPEAFTLEH VAKSVAYDRS SAPKDCRVSG SLQGPESSAE TENMQLLTEF TYDLDRSNAQ TFNILESSSS GLIDTVRLDF TSNHGSDSHT CIYRFRVHGR APDPVPVVGT NLDQDSSPES E ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-