At4g32060.1

In the plant Arabidopsis thaliana, At4g32060.1 is a gene model for the locus At4g32060.^[1]

This gene model is classified by TAIR as "calcium-binding EF hand family protein". It is located on chr4, region 15501140-15503327 FORWARD.

Physiological function[]

At4g32060.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At4g32060.1/Expression

Subcellular localization[]

At4g32060.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At4g32060.1 has 498 amino-acid residues and a molecular weight Mw = 55.418 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 4.89, or 0.010 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At4g32060.1/Knockout

Protein interactions[]

At4g32060.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At4g32060.1 has 3 recognized domains:

Domain_0213_0241 efhand domain (PF00036, IPR002048) Description: Calcium-binding EF-hand;Molecular Function: calcium ion binding (GO:0005509)

Domain_0247_0275 efhand domain (PF00036, IPR002048) Description: Calcium-binding EF-hand;Molecular Function: calcium ion binding (GO:0005509)

Domain_0441_0469 efhand domain (PF00036, IPR002048) Description: Calcium-binding EF-hand;Molecular Function: calcium ion binding (GO:0005509)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At4g32060.1 MPALSHYRSV SSLPSVDRSF LLIQRLRIHG SSSSFPESSP SASILSGADP LKCTVSGGSL AKWITGISAG SALGFLYWSS GSSDSISGLF GGSNLLSFAD SSTPSVCGVK VGDLKPRSFI PKLSLPGYSS GFIFGDAYRR KIFFNYEKRL RLQSPPEKVF EYFASVRTDK GEILMKPADL MRAIVPVFPP SESHLVREGY LTGERNPGEL RCSPSEFFML FDVDNDGLIS FKEYIFFVTL LSIPESSFAV AFKMFDTDNN GEIDKEEFKT VMSLMRSQHR QGVGHRDGLR TGLHMTGSVE DGGLVEYFFG KDGSQKLKHD KFTKFMKDLT EEMLRLEFAH YDYKRRGSIS AKDFALSMVA AADASHLSKL LDRVESLSEH PHLRDMRISL KEFKQFDELR SKLGPFSLAL FAYGKANGLL TMKDFKRAAS QVCGITLSDN VIEIAFHVFD SNQDGNLSVD EFLRVLHRRE RDVAQPIAKG LSRYFSDGWK GSKNCSSS ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-