At4g27600.1
From Proteins Wiki
In the plant Arabidopsis thaliana, At4g27600.1 is a gene model for the locus At4g27600.[1]
This gene model is classified by TAIR as "pfkB-type carbohydrate kinase family protein". It is located on chr4, region 13782759-13785011 REVERSE.
Contents |
[edit] Physiological function
At4g27600.1/Function NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
[edit] Tissue expression
[edit] Subcellular localization
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
[edit] Biophysical properties
The gene model At4g27600.1 has 471 amino-acid residues and a molecular weight Mw = 50.018 kDa.
The protein is relatively acidic; at the physiological pH 7.4, its net charge is -14.12, or -0.030 per residue. NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
[edit] Knockout phenotype
[edit] Protein interactions
At4g27600.1/Interactions This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
[edit] Domain structure
The gene model At4g27600.1 has 1 recognized domains:
- Domain_0200_0449 PfkB domain (PF00294, IPR011611) Description: PfkB
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
[edit] Amino-acid sequence
>At4g27600.1
MAFSLLSPIP
TSASLFFTLS
AITASNNASS
SSLSRLYSLY
HPKGTLRTRT
ATSFFGASGN
LRIVSMAENR
QLVCRIGGGA
GAELDDMDEG
EIQRIGNEDE
DDDEFIQVHA
NNSSAASPER
WDVLGLGQAM
VDFSGVVDDE
FLKKLGLEKG
TRKLINHEER
GKLLQAMDGC
SYKAAAGGSL
SNTLVALARL
GSQSICDRPL
NVAMAGSIGG
DPLGSFYGTK
LRRANVNFLS
APIKDGTTGT
VIVLTTPDAQ
RTMLAYQGTS
SVVNYDSCLA
SLIAKTNVFV
VEGYLFELPD
TIRTITKACE
EAHRNGALVA
VTASDVSCIE
RHYDDFWDIV
GNYADIVFAN
SDEARAFCHF
SAEESPISAT
RYMSHFVPFV
SVTDGINGSY
IGVKGEAIYI
PPSPCVPVDT
CGAGDAYASG
ILYGILRGVS
DLKGMGDMAA
TIAATVVGQQ
GTRLRVQDAV
ELARSHAFRL
NGSGVRTDVG
S
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
[edit] Multiple sequence alignment
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
[edit] Protein structure
A predicted structure for this protein is available here.
[edit] References
- ↑ This is a test reference.
