At4g26890.1
From Proteins Wiki
In the plant Arabidopsis thaliana, At4g26890.1 is a gene model for the locus At4g26890.[1]
Also known as MAPKKK16, this gene model is classified by TAIR as "MAPKKK16 (Mitogen-activated protein kinase kinase kinase 16); kinase". It is located on chr4, region 13512078-13513412 FORWARD.
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[edit] Physiological function
At4g26890.1/Function NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
[edit] Tissue expression
[edit] Subcellular localization
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
[edit] Biophysical properties
The gene model At4g26890.1 has 444 amino-acid residues and a molecular weight Mw = 49.537 kDa.
The protein is relatively acidic; at the physiological pH 7.4, its net charge is -31.54, or -0.071 per residue. NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
[edit] Knockout phenotype
[edit] Protein interactions
At4g26890.1/Interactions This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
[edit] Domain structure
The gene model At4g26890.1 has 1 recognized domains:
- Domain_0005_0253 Pkinase domain (PF00069, IPR000719) Description: Protein kinase;Molecular Function: protein kinase activity (GO:0004672), Molecular Function: ATP binding (GO:0005524), Biological Process: protein amino acid phosphorylation (GO:0006468)
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
[edit] Amino-acid sequence
>At4g26890.1
MEINWTRGPI
IGRGSTATVS
IAISSSGELF
AVKSADLSSS
SLLQKEQSIL
STLSSPHMVK
YIGTGLTRES
NGLVYNILME
YVSGGNLHDL
IKNSGGKLPE
PEIRSYTRQI
LNGLVYLHER
GIVHCDLKSH
NVLVEENGVL
KIADMGCAKS
VDKSEFSGTP
AFMAPEVARG
EEQRFPADVW
ALGCTMIEMM
TGSSPWPELN
DVVAAMYKIG
FSGESPAIPA
WISDKAKDFL
KNCLKEDQKQ
RWTVEELLKH
PFLDDDEESQ
TSDCLKNKTS
SPSTVLDQRF
WDSCESSKSH
LVSIDHEDPF
AEYSESLDSP
ADRIEKLAGD
EFSSLLDWDT
EDDGGWIQVR
GEKHKETEKR
DGDEDVICVE
ATSSSQTIEE
VEDWISNQDS
LFSEYSSDDI
INNFYSNVAI
QGNLIAFHYC
TDEGENVSIK
NMFRTNNKHL
FFKLQAHVKF
TNQV
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
[edit] Multiple sequence alignment
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
[edit] Protein structure
A predicted structure for this protein is available here.
[edit] References
- ↑ This is a test reference.
