At4g20070.1

In the plant Arabidopsis thaliana, At4g20070.1 is a gene model for the locus At4g20070.^[1]

Also known as ATAAH, this gene model is classified by TAIR as "ATAAH (ARABIDOPSIS THALIANA ALLANTOATE AMIDOHYDROLASE); allantoate deiminase/ metallopeptidase". It is located on chr4, region 10861558-10864539 FORWARD.

Physiological function[]

At4g20070.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At4g20070.1/Expression

Subcellular localization[]

At4g20070.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At4g20070.1 has 525 amino-acid residues and a molecular weight Mw = 56.504 kDa.

The protein is relatively acidic; at the physiological pH 7.4, its net charge is -16.98, or -0.032 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At4g20070.1/Knockout

Protein interactions[]

At4g20070.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At4g20070.1 has 2 recognized domains:

Domain_0163_0523 Peptidase_M20 domain (PF01546, IPR002933) Description: Peptidase M20;Biological Process: proteolysis (GO:0006508), Molecular Function: metallopeptidase activity (GO:0008237)

Domain_0299_0423 M20_dimer domain (PF07687, IPR011650) Description: Peptidase dimerisation;Molecular Function: hydrolase activity (GO:0016787), Molecular Function: protein dimerization activity (GO:0046983)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At4g20070.1 MAVPHPSSSS SRSHPFLSHV YHTSFHHHHH HNHPSLVLFW CLVFSLLSPL ALSSSSSSSS SSSDSSSSSS SHISLGIGET EGTKHDLHQA ILRDEAVARL HELGQVSDAA THLERTFMSP ASIRAIPLIR GWMEDAGLST WVDYMGNVHG RVEPKNGSSQ ALLIGSHMDT VIDAGKYDGS LGIISAISAL KVLKIDGRLG ELKRPVEVIA FSDEEGVRFQ STFLGSAALA GIMPVSRLEV TDKSGISVQD ALKENSIDIT DENLMQLKYD PASVWGYVEV HIEQGPVLEW VGYPLGVVKG IAGQTRLKVT VKGSQGHAGT VPMSMRQDPM TGAAELIVLL ESVCKNPKDY LSCNVQCNED TVESLANSLV CTVGEISTWP SASNVIPGQV TFTVDLRTID DVGRKAILHD LSTRMYQICD KRSLLCSIER KHDADAVMSD PQLSLQLKSA AQSALKKMTG EVQDEVPVLM SGAGHDAMAM AHLTKVGMLF VRCRGGISHS PAEHVLDDDV GAAGLAILEF LESQM ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-