In the plant Arabidopsis thaliana, At4g10500.1 is a gene model for the locus At4g10500.[1]
This gene model is classified by TAIR as "oxidoreductase, 2OG-Fe(II) oxygenase family protein". It is located on chr4, region 6491085-6492338 FORWARD.
Physiological function[]
At4g10500.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At4g10500.1/Expression
Subcellular localization[]
At4g10500.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At4g10500.1 has 349 amino-acid residues and a molecular weight Mw = 39.223 kDa.
The protein is relatively acidic; at the physiological pH 7.4, its net charge is -10.11, or -0.029 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At4g10500.1/Knockout
Protein interactions[]
At4g10500.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At4g10500.1 has 1 recognized domains:
- Domain_0197_0296 2OG-FeII_Oxy domain (PF03171, IPR005123) Description: 2OG-Fe(II) oxygenase_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At4g10500.1
MATSAISKLL
VSDFASSVHI
PSNYVRPISD
RPNLSEVESS
GDSIPLIDLR
DLHGPNRAVI
VQQLASACST
YGFFQIKNHG
VPDTTVNKMQ
TVAREFFHQP
ESERVKHYSA
DPTKTTRLST
SFNVGADKVL
NWRDFLRLHC
FPIEDFIEEW
PSSPISFREV
TAEYATSVRA
LVLRLLEAIS
ESLGLESDHI
SNILGKHAQH
MAFNYYPPCP
EPELTYGLPG
HKDPTVITVL
LQDQVSGLQV
FKDDKWVAVS
PIPNTFIVNI
GDQMQVISND
KYKSVLHRAV
VNTENERLSI
PTFYFPSTDA
VIGPAHELVN
EQDSLAIYRT
YPFVEYWDKF
WNRSLATASC
LDAFKAPTT
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.