At3g53460.2

From Proteins Wiki

In the plant Arabidopsis thaliana, At3g53460.2 is a gene model for the locus At3g53460.^[1]

Also known as CP29, this gene model is classified by TAIR as "CP29 (chloroplast 29 kDa ribonucleoprotein); RNA binding". It is located on chr3, region 19830716-19832401 REVERSE.

Contents 1 Physiological function 2 Tissue expression 3 Subcellular localization 4 Biophysical properties 5 Knockout phenotype 6 Protein interactions 7 Domain structure 8 Amino-acid sequence 9 Multiple sequence alignment 10 Protein structure 11 References 12 Further reading 13 General database links

[edit] Physiological function

At3g53460.2/Function NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

[edit] Tissue expression

At3g53460.2/Expression

[edit] Subcellular localization

At3g53460.2/Localization

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

[edit] Biophysical properties

The gene model At3g53460.2 has 334 amino-acid residues and a molecular weight Mw = 35.237 kDa.

The protein is relatively acidic; at the physiological pH 7.4, its net charge is -6.00, or -0.018 per residue. NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

[edit] Knockout phenotype

At3g53460.2/Knockout

[edit] Protein interactions

At3g53460.2/Interactions This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

[edit] Domain structure

The gene model At3g53460.2 has 2 recognized domains:

• Domain_0101_0172 RRM_1 domain (PF00076, IPR000504) Description: RNA-binding region RNP-1 (RNA recognition motif);Molecular Function: nucleic acid binding (GO:0003676)

• Domain_0251_0322 RRM_1 domain (PF00076, IPR000504) Description: RNA-binding region RNP-1 (RNA recognition motif);Molecular Function: nucleic acid binding (GO:0003676)

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

[edit] Amino-acid sequence

>At3g53460.2
MSASASSLSA FNPKSLPLCV SRPASVSVLP PSLSFKLHSD HLVSIFASSA LKCSSPAEYP SRFVRNVAVS SDFEVEEDDM FADGDDSAPV ERNSFSPDLK LFVGNLSFNV DSAQLAQLFE SAGNVEMVEV IYDKVTGRSR GFGFVTMSTA AEVEAAAQQF NGYEFEGRPL RVNAGPPPPK REESFSRGPR SGGYGSERGG GYGSERGGGY GSERGGGYGS QRSGGGYGGS QRSSYGSGSG SGSGSGSGNR LYVGNLSWGV DDMALENLFN EQGKVVEARV IYDRDSGRSK GFGFVTLSSS QEVQKAINSL NGADLDGRQI RVSEAEARPP RGQF MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-

[edit] Multiple sequence alignment

A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.

[edit] Protein structure

A predicted structure for this protein is available here.

[edit] References

[edit] Further reading

• PubMed references for At3g53460

• PubMed Central references for At3g53460

• Google Scholar references for At3g53460

[edit] General database links