At3g53460.2

From Proteins Wiki

In the plant Arabidopsis thaliana, At3g53460.2 is a gene model for the locus At3g53460.^[1]

Also known as CP29, this gene model is classified by TAIR as "CP29 (chloroplast 29 kDa ribonucleoprotein); RNA binding". It is located on chr3, region 19830716-19832401 REVERSE.

[edit] Physiological function

At3g53460.2/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

[edit] Tissue expression

At3g53460.2/Expression

[edit] Subcellular localization

At3g53460.2/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

[edit] Biophysical properties

The gene model At3g53460.2 has 334 amino-acid residues and a molecular weight Mw = 35.237 kDa.

The protein is relatively acidic; at the physiological pH 7.4, its net charge is -6.00, or -0.018 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

[edit] Knockout phenotype

At3g53460.2/Knockout

[edit] Protein interactions

At3g53460.2/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

[edit] Domain structure

The gene model At3g53460.2 has 2 recognized domains:

Domain_0101_0172 RRM_1 domain (PF00076, IPR000504) Description: RNA-binding region RNP-1 (RNA recognition motif);Molecular Function: nucleic acid binding (GO:0003676)

Domain_0251_0322 RRM_1 domain (PF00076, IPR000504) Description: RNA-binding region RNP-1 (RNA recognition motif);Molecular Function: nucleic acid binding (GO:0003676)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

[edit] Amino-acid sequence

>At3g53460.2 MSASASSLSA FNPKSLPLCV SRPASVSVLP PSLSFKLHSD HLVSIFASSA LKCSSPAEYP SRFVRNVAVS SDFEVEEDDM FADGDDSAPV ERNSFSPDLK LFVGNLSFNV DSAQLAQLFE SAGNVEMVEV IYDKVTGRSR GFGFVTMSTA AEVEAAAQQF NGYEFEGRPL RVNAGPPPPK REESFSRGPR SGGYGSERGG GYGSERGGGY GSERGGGYGS QRSGGGYGGS QRSSYGSGSG SGSGSGSGNR LYVGNLSWGV DDMALENLFN EQGKVVEARV IYDRDSGRSK GFGFVTLSSS QEVQKAINSL NGADLDGRQI RVSEAEARPP RGQF ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-