At3g03940.1

In the plant Arabidopsis thaliana, At3g03940.1 is a gene model for the locus At3g03940.

This gene model is classified by TAIR as "protein kinase family protein". It is located on chr3, region 1014419-1018251 REVERSE.

Physiological function

At3g03940.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression

At3g03940.1/Expression

Subcellular localization

At3g03940.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties

The gene model At3g03940.1 has 701 amino-acid residues and a molecular weight Mw = 78.008 kDa.

The protein is relatively basic; at the physiological pH 7.4, its net charge is 15.21, or 0.022 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype

At3g03940.1/Knockout

Protein interactions

At3g03940.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure

The gene model At3g03940.1 has 1 recognized domains:

• Domain_0140_0374 Pkinase domain (PF00069, IPR000719) Description: Protein kinase;Molecular Function: protein kinase activity (GO:0004672), Molecular Function: ATP binding (GO:0005524), Biological Process: protein amino acid phosphorylation (GO:0006468)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence

>At3g03940.1
MPELRSGARR SRRLDEQPNP PLVEQAENIV LPPQTATRRR GGGRGRGNAA LAKGAAPPRP TAAGRGRGIR LTDLEPEPCE VRPAAGAIGA TEPALNRVEG VADKDIAAEG GSAEKVVGME EDSSMGPVPE RVQVGNSPVY KTERKLGKGG FGQVYVGRRV SGGSDRIGAD AIEVALKLEH RNSKGCNFGP PYEWQVYNTL NSCYGIPAVH HKGRQGDFYI LVMDMLGPSL WDVWNSLAQS MSPNMVACIA VEAISILEKL HMKGFVHGDV KPENFLLGQP GTADEKKLYL IDLGLASRWK DSHSGQHVEY DQRPDVFRGT IRYASCHAHL GRTGSRRDDL ESLAYTLIFL MRGRLPWQGY QGDNKSFLVC KKKMSTSPEL MCCFCPPPFK LFLEAVTNMK FDEEPNYAKL ISIFDTLIEP CAISRPIRID GALKVGQKRG RLLINLEEDE QPRKKIRIGS PATQWISVYN ARRPMKQRYH YNVADLRLAQ HVEKGNEDGL FISCVASSAN LWAIIMDAGT GFSSQVYELS SVFLHKDWIM EQWEKNYYIS SIAGANNGSS LVVMAKGTPY TQQSYKVSDS FPFKWINKKW KEGFHVTSMT TAGSRWGVVM SRNSGYSEQV VELDFLYPSE GIHRRWESGY RITSMAATAD QAALILSIPK RKITDETQET LRTSAFPSTH VKEKWAKNLY IASICYGRTV C ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-

Multiple sequence alignment

A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.

Protein structure

A predicted structure for this protein is available here.

Further reading

• PubMed references for At3g03940

• PubMed Central references for At3g03940

• Google Scholar references for At3g03940

General database links

• TAIR Locus At3g03940

• TIGR page for At3g03940

• KEGG page for At3g03940

• SWISS-PROT entry for At3g03940

• Structural model of At3g03940

• NCBI structural data for At3g03940

• NCBI Entrez for At3g03940

• Gramene page for At3g03940

• MIPS MAtDB gene page for At3g03940

• MIPS SESAM alignment for At3g03940

• MIPS PENDANT general properties

• MIPS PENDANT general sequence alignment

• NCBI gene data for At3g03940

• NCBI protein data for At3g03940

• NCBI location on chromosome

• SUBA subcellular localization

• Arabidopsis MPSS Plus on At3g03940