In the plant Arabidopsis thaliana, At3g03700.1 is a gene model for the locus At3g03700.[1]
This gene model is classified by TAIR as "similar to unknown protein [Arabidopsis thaliana] (TAIR:AT3G04440.1); similar to Protein of unknown function DUF580 [Medicago truncatula] (GB:ABE92417.1); contains InterPro domain Protein of unknown function DUF580; (InterPro:IPR007603)". It is located on chr3, region 916510-918334 FORWARD.
Physiological function[]
At3g03700.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At3g03700.1/Expression
Subcellular localization[]
At3g03700.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At3g03700.1 has 482 amino-acid residues and a molecular weight Mw = 53.063 kDa.
The protein is relatively basic; at the physiological pH 7.4, its net charge is 8.98, or 0.019 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At3g03700.1/Knockout
Protein interactions[]
At3g03700.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At3g03700.1 has 1 recognized domains:
- Domain_0115_0470 DUF580 domain (PF04515, IPR007603) Description: Protein of unknown function DUF580_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At3g03700.1
MPTPTSNGHA
SRVQIQEPRI
MSPLPPSSSP
IAFKEQQGRP
PPTTQQTIAG
KLFRTLFKGL
LFSQLTLISL
LVIVLTIRGL
ISASTHHFHL
KKWYPPLLAS
VAVSGIASLA
WQCIFIYNPS
RAVKATFWLS
PILTCSVGIL
LVLIGSAVDA
GIGAVFVLFA
ITQSLYGCWI
TPRLEYTDKI
LSLATAFPPA
RTREVVCLSI
IVSVVYSGFL
VTGIGGATST
RTNLDILFIS
VIIISLAWTM
QVIKNVQQVA
ISRARYVNFA
HGEDMDAWNA
FRITMKHLTG
SICVGSTLVP
IIVFIRGSIR
SVNLMSGSSD
EVMYSGADCF
STIANKLITL
GNRWGFVHVG
TYDKGFMEAS
SDTWKKFRST
TGLEKLIDSD
LTSSFCFLSA
VSVGAVSSLT
AGIWMLLIHK
DYALEVTLYA
FIIGYFVGRV
GLAWLQACVL
AYYVAYSEDP
QSMRFDGTIP
QRIQRLQMLS
AHRDNRELEV
GRDREEDSYN
NC
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.