At2g43680.1

From Proteins Wiki

In the plant Arabidopsis thaliana, At2g43680.1 is a gene model for the locus At2g43680.^[1]

At2g43680.1/Header

Contents 1 Physiological function 2 Tissue expression 3 Subcellular localization 4 Biophysical properties 5 Knockout phenotype 6 Protein interactions 7 Domain structure 8 Amino-acid sequence 9 Multiple sequence alignment 10 Protein structure 11 References 12 Further reading 13 General database links

[edit] Physiological function

At2g43680.1/Function NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

[edit] Tissue expression

At2g43680.1/Expression

[edit] Subcellular localization

At2g43680.1/Localization

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

[edit] Biophysical properties

At2g43680.1/Biophysics NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

[edit] Knockout phenotype

At2g43680.1/Knockout

[edit] Protein interactions

At2g43680.1/Interactions This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

[edit] Domain structure

At2g43680.1/Domains NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

[edit] Amino-acid sequence

>At2g43680.1
MVKKGSWFSA IKRVFTPHSK EKLANEPERK SGKEKKKKGF GKLRHGETNS FLPIFREPSS IEKILGEAER DHNLVFRPPT PDRPNPYSAS PPPRPASPRV ASPRPTSPRV ASPRVPSPRA EVPRTLSPKP PSPRAEVPRS LSPKPPSPRA DLPRSLSPKP FDRSKPSSAS ANAPPTLRPA STRVPSQRIT PHSVPSPRPS SPRGASPQAI SSKPPSPRAE PPTLDTPRPP SPRAASLRAD PPRLDAARPT TPRPPSPLAD APRLDAPRPT TPKPPSPRSD PPRLDAPRPT TPKPPSPRSV SPRAVQRREI VYRPEPTLPV QHASATKIQG AFRGYMARKS FRALKGLVRL QGVVRGYSVK RQTINAMKYM QQVVRVQSQI QSRRIKMLEN QAQVEKDEAK WAASEAGNDN WDDSVLTKEE RDSRSQRKTD AIIKRERSMA YAYSRKLWKN SPKSTQDNRS FPQWWNWVDR QNPLASPAPS YSQPQRDFRL TPSRLCPSPL SQSSKQHHIR LDNHFDTSTP RSSRSTFHTP SRPIHTGTSR YSRGRLRGQD SPFKDDDSLT SCPPFPSYMA PTVSAKAKVR PNSNPKERVM GTPVSEKRRM SYPPTQDTFR WNKGSLVMSN SSSHRGPGSP GGVVLEKHKT LKSVGNLSIG STASMATTVG RKEFNRFV MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-

[edit] Multiple sequence alignment

A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.

[edit] Protein structure

A predicted structure for this protein is available here.

[edit] References

[edit] Further reading

• PubMed references for At2g43680

• PubMed Central references for At2g43680

• Google Scholar references for At2g43680

[edit] General database links