At2g36390.1

In the plant Arabidopsis thaliana, At2g36390.1 is a gene model for the locus At2g36390.

Also known as BE3, and SBE2.1, this gene model is classified by TAIR as "SBE2.1 (STARCH BRANCHING ENZYME 2.1); 1,4-alpha-glucan branching enzyme". It is located on chr2, region 15271362-15277019 FORWARD.

Physiological function

At2g36390.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression

At2g36390.1/Expression

Subcellular localization

At2g36390.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties

The gene model At2g36390.1 has 858 amino-acid residues and a molecular weight Mw = 97.626 kDa.

The protein is relatively acidic; at the physiological pH 7.4, its net charge is -29.57, or -0.034 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype

At2g36390.1/Knockout

Protein interactions

At2g36390.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure

The gene model At2g36390.1 has 2 recognized domains:

• Domain_0204_0288 Isoamylase_N domain (PF02922, IPR004193) Description: Glycoside hydrolase, family 13, N-terminal;Molecular Function: hydrolase activity, hydrolyzing O-glycosyl compounds (GO:0004553), Biological Process: carbohydrate metabolism (GO:0005975)

• Domain_0356_0417 Alpha-amylase domain (PF00128, IPR006047) Description: Alpha amylase, catalytic region;Molecular Function: alpha-amylase activity (GO:0004556), Biological Process: carbohydrate metabolism (GO:0005975)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence

>At2g36390.1
MVYTISGVRF PHLPSIKKKN SSLHSFNEDL RRSNAVSFSL RKDSRSSGKV FARKPSYDSD SSSLATTASE KLRGHQSDSS SSASDQVQSR DTVSDDTQVL GNVDVQKTEE AQETETLDQT SALSTSGSIS YKEDFAKMSH SVDQEVGQRK IPPPGDGKRI YDIDPMLNSH RNHLDYRYGQ YRKLREEIDK NEGGLEAFSR GYEIFGFTRS ATGITYREWA PGAKAASLIG DFNNWNAKSD VMARNDFGVW EIFLPNNADG SPAIPHGSRV KIRMDTPSGI KDSIPAWIKY SVQPPGEIPY NGVYYDPPEE DKYAFKHPRP KKPTSLRIYE SHVGMSSTEP KINTYANFRD DVLPRIKKLG YNAVQIMAIQ EHAYYASFGY HVTNFFAPSS RFGTPDDLKS LIDKAHELGL VVLMDIVHSH ASKNTLDGLD MFDGTDGQYF HSGSRGYHWM WDSRLFNYGS WEVLRYLLSN ARWWLEEYKF DGFRFDGVTS MMYTHHGLQV EFTGNYNEYF GYSTDVDAVV YLMLVNDLIH GLYPEAIVVG EDVSGMPAFC VPVEDGGVGF DYRLHMAVAD KWIELLKKRD EDWQVGDITF TLTNRRWGEK CVVYAESHDQ ALVGDKTIAF WLMDKDMYDF MAVDRQATPR VDRGIALHKM IRLITMGLGG EGYLNFMGNE FGHPEWIDFP RTDQHLPDGR VIAGNNGSYD KCRRRFDLGD AEYLRYHGLQ EFDRAMQNLE ETYGFMTSEH QYISRKDEGD RVIVFERGNL LFVFNFHWTN SYSDYRIGCS VPGKYKIVLD SDNSLFGGFN RLDDSAEFFT SDGRHDDRPC SFMVYAPCRT AVVYAAVDDD DDDERSSLVP IGLLPEDV ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-

Multiple sequence alignment

A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.

Protein structure

A predicted structure for this protein is available here.

Further reading

• PubMed references for At2g36390

• PubMed Central references for At2g36390

• Google Scholar references for At2g36390

General database links

• TAIR Locus At2g36390

• TIGR page for At2g36390

• KEGG page for At2g36390

• SWISS-PROT entry for At2g36390

• Structural model of At2g36390

• NCBI structural data for At2g36390

• NCBI Entrez for At2g36390

• Gramene page for At2g36390

• MIPS MAtDB gene page for At2g36390

• MIPS SESAM alignment for At2g36390

• MIPS PENDANT general properties

• MIPS PENDANT general sequence alignment

• NCBI gene data for At2g36390

• NCBI protein data for At2g36390

• NCBI location on chromosome

• SUBA subcellular localization

• Arabidopsis MPSS Plus on At2g36390