In the plant Arabidopsis thaliana, At2g35060.2 is a gene model for the locus At2g35060.[1]
At2g35060.2/Header
Physiological function[]
At2g35060.2/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At2g35060.2/Expression
Subcellular localization[]
At2g35060.2/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
At2g35060.2/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At2g35060.2/Knockout
Protein interactions[]
At2g35060.2/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
At2g35060.2/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At2g35060.2
MAARVEAATM
GGEIDEEESD
ERGSMWDLDQ
KLDQSMDEEA
GRLRNMYREK
KFSALLLLQL
SFQSLGVVYG
DLGTSPLYVF
YNTFPHGIKD
PEDIIGALSL
IIYSLTLIPL
LKYVFVVCKA
NDNGQGSGTF
ALYSLLCRHA
KVKTIQNQHR
TDEELTTYSR
TTFHEHSFAA
KTKRWLEKRT
SRKTALLILV
LVGTCMVIGD
GILTPAISVL
SAAGGLRVNL
PHISNGVVVF
VAVVILVSLF
SVQHYGTDRV
GWLFAPIVFL
WFLSIASIGM
YNIWKHDTSV
LKAFSPVYIY
RYFKRGGRDR
WTSLGGIMLS
ITGIEALFAD
LSHFPVSAVQ
IAFTVIVFPC
LLLAYSGQAA
YIRRYPDHVA
DAFYRSIPGS
VYWPMFIIAT
AAAIVASQAT
ISATFSLVKQ
ALAHGCFPRV
KVVHTSRKFL
GQIYVPDINW
ILMILCIAVT
AGFKNQSQIG
NAYGTAVVIV
MLVTTLLMTL
IMILVWRCHW
VLVLIFTVLS
LVVECTYFSA
MLFKIDQGGW
VPLVIAAAFL
LIMWVWHYGT
LKRYEFEMHC
RVSMAWILGL
GPSLGLVRVP
GVGLVYTELA
SGVPHIFSHF
ITNLPAIHSV
VVFVCVKNLP
VYTVPEEERF
LVKRIGPKNF
HMFRCVARYG
YRDLHKKDDD
FEKRLFESLF
LYVRLESMME
GGCSDSDDYS
ICGSQQQLKD
TLGNGNENEN
LATFDTFDSI
ESITPVKRVS
NTVTASSQMS
GVDELEFING
CRDAGVVHIM
GNTVVRARRE
ARFYKKIAID
YVYAFLRKIC
REHSVIYNVP
QESLLNVGQI
FYV
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.