At2g32480.1

In the plant Arabidopsis thaliana, At2g32480.1 is a gene model for the locus At2g32480.^[1]

This gene model is classified by TAIR as "membrane-associated zinc metalloprotease, putative". It is located on chr2, region 13795756-13797099 REVERSE.

Physiological function[]

At2g32480.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At2g32480.1/Expression

Subcellular localization[]

At2g32480.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At2g32480.1 has 447 amino-acid residues and a molecular weight Mw = 48.728 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 2.78, or 0.006 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At2g32480.1/Knockout

Protein interactions[]

At2g32480.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At2g32480.1 has 1 recognized domains:

Domain_0090_0436 Peptidase_M50 domain (PF02163, IPR008915) Description: Peptidase M50;Molecular Function: metalloendopeptidase activity (GO:0004222), Biological Process: proteolysis (GO:0006508)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At2g32480.1 MLLNISSSPI SHRNPHFLSN FNNPISYFPR RSKTHLSKSH FFPKFTPLSN QSLKNRVLFG NKRYPDGERF DFRSRAISGI DLGSFESVLE AIAVLTTIIV VHESGHFLAA SLQGIHVSKF AIGFGPILAK FDYNNVEYSL RAFPLGGFVG FPDNDPDSEI PIDDENLLKN RPTLDRSIVV SAGIIANVIF AYAIIFVQVL SVGLPVQEAF PGVLVPEVKT FSAASRDGLL SGDVILAVDG TELSKTGPDA VSKIVDIVKR NPKSNVVFRI ERGGEDFDIR VTPDKNFDGT GKIGVQLSPN VRITKVRPRN IPETFRFVGR EFMGLSSNVL DGLKQTFFNF SQTASKVAGP VAIIAVGAEV ARSNIDGLYQ FAALLNINLA VINLLPLPAL DGGTLALILL EAVRGGKKLP VEVEQGIMSS GIMLVIFLGL FLIVKDTLSL DFIKEML ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-