At2g26530.2

From Proteins Wiki

In the plant Arabidopsis thaliana, At2g26530.2 is a gene model for the locus At2g26530.^[1]

Also known as AR781, this gene model is classified by TAIR as "AR781". It is located on chr2, region 11290114-11291067 REVERSE.

Contents 1 Physiological function 2 Tissue expression 3 Subcellular localization 4 Biophysical properties 5 Knockout phenotype 6 Protein interactions 7 Domain structure 8 Amino-acid sequence 9 Multiple sequence alignment 10 Protein structure 11 References 12 Further reading 13 General database links

[edit] Physiological function

At2g26530.2/Function NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

[edit] Tissue expression

At2g26530.2/Expression

[edit] Subcellular localization

At2g26530.2/Localization

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

[edit] Biophysical properties

The gene model At2g26530.2 has 290 amino-acid residues and a molecular weight Mw = 33.130 kDa.

The protein is relatively basic; at the physiological pH 7.4, its net charge is 9.45, or 0.033 per residue. NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

[edit] Knockout phenotype

At2g26530.2/Knockout

[edit] Protein interactions

At2g26530.2/Interactions This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

[edit] Domain structure

The gene model At2g26530.2 has 1 recognized domains:

• Domain_0089_0281 DUF1645 domain (PF07816, IPR012442) Description: Protein of unknown function DUF1645

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

[edit] Amino-acid sequence

>At2g26530.2
MEVMSLTAPS SPRQLSGCFL SAPTSPRRFN EFYREFEEAA TRNFSDRLTV PFDWEETPGT PRKITNDDDD DIDFAFEIGG KLETTSLFAE ELFDGGKIKP LKPPPYLQLD HHHQPQILSP RSPRSPIAHA RNGLGEERGR GRRQNSGRRV ARSLSPFRVS AYPWEEQEQE QEQEQRDVQE QRKGTLSSIP STSSSACVSC KSSSSKKWRL KDFLLFRSAS EGRARHNKDS VKTFTSLFRK QEDTKNSSSR GRGSSSVSAH EFHYMSKKAE TKDLKKKTFL PYMQIGRFAF MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-

[edit] Multiple sequence alignment

A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.

[edit] Protein structure

A predicted structure for this protein is available here.

[edit] References

[edit] Further reading

• PubMed references for At2g26530

• PubMed Central references for At2g26530

• Google Scholar references for At2g26530

[edit] General database links