In the plant Arabidopsis thaliana, At2g14910.2 is a gene model for the locus At2g14910.[1]
This gene model is classified by TAIR as "similar to unknown protein [Arabidopsis thaliana] (TAIR:AT5G14970.1); similar to seed maturation protein PM23 [Glycine max] (GB:AAF21309.1)". It is located on chr2, region 6414277-6416097 REVERSE.
Physiological function[]
At2g14910.2/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At2g14910.2/Expression
Subcellular localization[]
At2g14910.2/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At2g14910.2 has 366 amino-acid residues and a molecular weight Mw = 40.997 kDa.
The protein is relatively acidic; at the physiological pH 7.4, its net charge is -15.54, or -0.042 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At2g14910.2/Knockout
Protein interactions[]
At2g14910.2/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At2g14910.2 has no known domains._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
MATTTLSSFSLSLPQLLHKPTKPLPFLFLLPRFNRRFRSLTITSSSTTSSNNFSSNCGDDGFSLDDFTLH SDSRSPKKCVLSDLIQEIEPLDVSLIQKDVPVTTLDAMKRTISGMLGLLPSDRFQVHIESLWEPLSKLLV SSMMTGYTLRNAEYRLFLEKNLDMSGGGLDSHASENTEYDMEGTFPDEDHVSSKRDSRTQNLSETIDEEG LGRVSSEAQEYILRLQSQLSSVKKELQEMRRKNAALQMQQFVGEEKNDLLDYLRSLQPEKVAELSEPAAP EVKETIHSVVHGLLATLSPKMHSKFPASEVPPTETVKAKSDEDCAELVENTSLQFQPLISLTRDYLARLL FWLEELPSSTSLSLAC ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.