At2g07750.1
From Proteins Wiki
In the plant Arabidopsis thaliana, At2g07750.1 is a gene model for the locus At2g07750.[1]
This gene model is classified by TAIR as "DEAD box RNA helicase, putative". It is located on chr2, region 3576480-3580393 FORWARD.
Contents |
[edit] Physiological function
At2g07750.1/Function NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
[edit] Tissue expression
[edit] Subcellular localization
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
[edit] Biophysical properties
The gene model At2g07750.1 has 845 amino-acid residues and a molecular weight Mw = 94.916 kDa.
The protein is relatively basic; at the physiological pH 7.4, its net charge is 32.12, or 0.038 per residue. NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
[edit] Knockout phenotype
[edit] Protein interactions
At2g07750.1/Interactions This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
[edit] Domain structure
The gene model At2g07750.1 has 2 recognized domains:
- Domain_0399_0579 DEAD domain (PF00270, IPR011545) Description: DEAD/DEAH box helicase, N-terminal;Molecular Function: nucleic acid binding (GO:0003676), Molecular Function: helicase activity (GO:0004386), Molecular Function: ATP binding (GO:0005524)
- Domain_0653_0729 Helicase_C domain (PF00271, IPR001650) Description: Helicase, C-terminal;Molecular Function: nucleic acid binding (GO:0003676), Molecular Function: helicase activity (GO:0004386), Molecular Function: ATP binding (GO:0005524)
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
[edit] Amino-acid sequence
>At2g07750.1
MAMAMRLPAI
SRAVTEVASS
PVGLRRLFCS
NASRFSFLSP
PARRQAEPST
NLFHSGLSKR
ITSERSLWNR
IFSRNMGGGP
RTFPGGLNKW
QWKRMHEKKA
REKENKLLDQ
EKQLYEARIR
TEIRAKMWGH
PDSGEKTAKL
KQSHGPMSPK
EHIKTLADRF
MKAGADDLWN
DNDGPVKKFD
QGSRSCSDSI
DSTPIDVRRL
VSATCDSMGK
HRVLDSSRRG
FSSMSRFKRN
ESSCDEGDDV
DAKKLDTLSP
FSPKFSGTKE
KVKSSTSVVG
VIRNKGLFGR
RKFRKNDSST
EEDSDEEGNE
GKMIGWMDLR
KTGSSASLGN
HDIKLTKRVN
RNVTDEELYP
PLDINRVRED
LSKKQSVDNV
MEEKQEPHDS
IYSAKRFDES
CISPLTLKAL
SASGIVKMTR
VQDATLSECL
DGKDALVKAK
TGTGKSMAFL
LPAIETVLKA
MNSGKGVHKV
APIFVLILCP
TRELASQIAA
EGKALLKNHD
GIGVQTLIGG
TRFRLDQQRL
ESEPCQILIA
TPGRLLDHIE
NKSGLTSRLM
ALKLFIVDEA
DLLLDLGFKR
DVEKIIDCLP
RQRQSLLFSA
TIPKEVRRVS
QLVLKRDHSY
IDTIGLGCVE
THDKVKQSCI
VAPHESHFHL
VPHLLKEHIN
NMPDYKIIVF
CSTGMVTSLM
YTLLREMKLN
VREIHARKPQ
LHRTCVSDEF
KESNRLILVT
SDVSARGMNY
PDVTLVIQVG
IPSDREQYIH
RLGRTGREGK
GGKGLLLIAP
WERYFLDELK
DLPLEPIPAP
DLDSRVKHQV
DQSMAKIDTS
IKEAAYHAWL
GYYNSVRETG
RDKTTLAELA
NRFCHSIGLE
KPPALFRRTA
VKMGLKGISG
IPIRK
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
[edit] Multiple sequence alignment
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
[edit] Protein structure
A predicted structure for this protein is available here.
[edit] References
- ↑ This is a test reference.
