At2g05590.1

In the plant Arabidopsis thaliana, At2g05590.1 is a gene model for the locus At2g05590.^[1]

This gene model is classified by TAIR as "similar to unknown protein [Arabidopsis thaliana] (TAIR:AT4G39870.1); similar to TLD, putative [Medicago truncatula] (GB:ABE87991.1); contains InterPro domain TLDc; (InterPro:IPR006571)". It is located on chr2, region 2067193-2068647 FORWARD.

Physiological function[]

At2g05590.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At2g05590.1/Expression

Subcellular localization[]

At2g05590.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At2g05590.1 has 263 amino-acid residues and a molecular weight Mw = 28.958 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is -0.68, or -0.003 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At2g05590.1/Knockout

Protein interactions[]

At2g05590.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At2g05590.1 has 1 recognized domains:

Domain_0158_0263 TLD domain (PF07534, IPR006571) Description: TLDc_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

MHALKDKVSQKLSNLFADSPSQSASPRYSNSDSPKARLNSSVGKSLSSYFSFVVPQSGNEEDSELCPPLP IRTESYECIENCKSANGQAKAGTFISIGEDKDCELRVSAKVEESGNDYFDGVKKMRELTESSVFITANLF EFLHASLPNIVRGCKWILLYSTLKHGISLRTLLRRSGELPGPCLLVAGDKQGAVFGALLECPLQPTPKRK YQGTSQTFLFTTIYGEPRIFRPTGANRYYLMCMNEFLAFGGGGNFALCLDEDL ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-