At2g05100.1

In the plant Arabidopsis thaliana, At2g05100.1 is a gene model for the locus At2g05100.^[1]

Also known as LHCB2.3, and LHCB2.1, this gene model is classified by TAIR as "LHCB2.1 (Photosystem II light harvesting complex gene 2.1); chlorophyll binding". It is located on chr2, region 1823446-1824328 REVERSE.

Physiological function[]

At2g05100.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At2g05100.1/Expression

Subcellular localization[]

At2g05100.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At2g05100.1 has 265 amino-acid residues and a molecular weight Mw = 28.644 kDa.

The protein is relatively acidic; at the physiological pH 7.4, its net charge is -4.79, or -0.018 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At2g05100.1/Knockout

Protein interactions[]

At2g05100.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At2g05100.1 has 1 recognized domains:

Domain_0064_0232 Chloroa_b-bind domain (PF00504, IPR001344) Description: Chlorophyll A-B binding protein;Biological Process: photosynthesis, light harvesting (GO:0009765), Cellular Component: membrane (GO:0016020)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At2g05100.1 MATSAIQQSS FAGQTALKPS NELLRKVGVS GGGRVTMRRT VKSTPQSIWY GPDRPKYLGP FSENTPSYLT GEYPGDYGWD TAGLSADPET FAKNRELEVI HSRWAMLGAL GCTFPEILSK NGVKFGEAVW FKAGSQIFSE GGLDYLGNPN LIHAQSILAI WAVQVVLMGF IEGYRIGGGP LGEGLDPLYP GGAFDPLNLA EDPEAFSELK VKELKNGRLA MFSMFGFFVQ AIVTGKGPIE NLFDHLADPV ANNAWSYATN FVPGN ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-