At2g03360.1

In the plant Arabidopsis thaliana, At2g03360.1 is a gene model for the locus At2g03360.^[1]

This gene model is classified by TAIR as "similar to serine carboxypeptidase [Arabidopsis thaliana] (TAIR:AT2G03370.1); similar to Os01g0498300 [Oryza sativa (japonica cultivar-group)] (GB:NP_001043125.1); similar to Os01g0956200 [Oryza sativa (japonica cultivar-group)] (GB:NP_001045438.1); similar to glycosyltransferase [Saccharum officinarum] (GB:CAI30073.1); contains InterPro domain Protein of unknown function DUF563; (InterPro:IPR007657)". It is located on chr2, region 1022284-1023465 REVERSE.

Physiological function[]

At2g03360.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At2g03360.1/Expression

Subcellular localization[]

At2g03360.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At2g03360.1 has 393 amino-acid residues and a molecular weight Mw = 44.460 kDa.

The protein is relatively basic; at the physiological pH 7.4, its net charge is 8.43, or 0.021 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At2g03360.1/Knockout

Protein interactions[]

At2g03360.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At2g03360.1 has 1 recognized domains:

Domain_0007_0253 DUF563 domain (PF04577, IPR007657) Description: Protein of unknown function DUF563_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At2g03360.1 MAASAKITCD RSHTSYDLCS INGSCILNPK TGTLTLMDRT LTTSAPLVEK IRPYPRKSEN WIMPRIRELK LTSGPSDLTR SCDITHDSPA IVFSAGGYTG SIYHDFIDGF IPLFITANSV YPDRDFILVV VNPKEWWMPK YIDILGTFSK HKTILLDKEN ASITHCFTSA TVGLISHGPM TIDPTQIPNS KSLVDFHNLL DKALNPNLSI IKINKPRLIL VRRYGNIGRV ILNEEEIREM LEDVGFEVIT FRPSKTTSLR EAYKLIKSSH GMIGVHGAAL TQLLFLRPGS VLVQIVPVGL GWVSKTCFET PAKAMKLDYT EYRVNVEESS LIEKYSRDDL VLKDPIAYRG MDWNVTKMKV YLKDQDVRLD VNRFRKHMNE AYKKAKSFMD LNG ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-