At2g01940.1

In the plant Arabidopsis thaliana, At2g01940.1 is a gene model for the locus At2g01940.^[1]

Also known as SGR5, and ATIDD15, this gene model is classified by TAIR as "SGR5 (SHOOT GRAVITROPISM 5); nucleic acid binding / transcription factor/ zinc ion binding". It is located on chr2, region 432651-434916 FORWARD.

Physiological function[]

At2g01940.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At2g01940.1/Expression

Subcellular localization[]

At2g01940.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At2g01940.1 has 445 amino-acid residues and a molecular weight Mw = 50.157 kDa.

The protein is relatively basic; at the physiological pH 7.4, its net charge is 17.33, or 0.039 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At2g01940.1/Knockout

Protein interactions[]

At2g01940.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At2g01940.1 has 2 recognized domains:

Domain_0073_0095 zf-C2H2 domain (PF00096, IPR007087) Description: Zinc finger, C2H2-type;Molecular Function: nucleic acid binding (GO:0003676), Cellular Component: nucleus (GO:0005634), Molecular Function: zinc ion binding (GO:0008270)

Domain_0151_0173 zf-C2H2 domain (PF00096, IPR007087) Description: Zinc finger, C2H2-type;Molecular Function: nucleic acid binding (GO:0003676), Cellular Component: nucleus (GO:0005634), Molecular Function: zinc ion binding (GO:0008270)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At2g01940.1 MRTDQVMLSN KNTNTCCVVS SSSSDPFLSS SENGVTTTNT STQKRKRRPA GTPDPDAEVV SLSPRTLLES DRYICEICNQ GFQRDQNLQM HRRRHKVPWK LLKRDNNIEV KKRVYVCPEP TCLHHNPCHA LGDLVGIKKH FRRKHSNHKQ WVCERCSKGY AVQSDYKAHL KTCGTRGHSC DCGRVFSRVE SFIEHQDNCS ARRVHREPPR PPQTAVTVPA CSSRTASTVS TPSSETNYGG TVAVTTPQPL EGRPIHQRIS SSILTNSSNN LNLELQLLPL SSNQNPNQEN QQQKVKEPSH HHNHNHDTTN LNLSIAPSSS YQHYNNFDRI KEIMASEQIM KIAMKEKAYA EEAKREAKRQ REIAENEFAN AKKIRQKAQA ELERAKFLKE QSMKKISSTI MQVTCQTCKG QFQAVAVPAA TADETSLVVS YMSSANTDGE LENGF ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-