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In the plant Arabidopsis thaliana, At2g01390.1 is a gene model for the locus At2g01390.[1]

This gene model is classified by TAIR as "pentatricopeptide (PPR) repeat-containing protein". It is located on chr2, region 172255-174136 FORWARD.

Physiological functionEdit

At2g01390.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expressionEdit

At2g01390.1/Expression

Subcellular localizationEdit

At2g01390.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical propertiesEdit

The gene model At2g01390.1 has 577 amino-acid residues and a molecular weight Mw = 65.603 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 1.55, or 0.003 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotypeEdit

At2g01390.1/Knockout

Protein interactionsEdit

At2g01390.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structureEdit

The gene model At2g01390.1 has 7 recognized domains:

  • Domain_0487_0521 PPR domain (PF01535, IPR002885) Description: Pentatricopeptide repeat_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequenceEdit

>At2g01390.1
MRFSSIFRNT PIQFSNRRSS VKLLHSLPRL KPTNSKRFSQ KPKLVKTQTL PDPSVYTRDI VSNIYNILKY SNWDSAQEQL PHLGVRWDSH IINRVLKAHP PMQKAWLFFN WAAQIKGFKH DHFTYTTMLD IFGEAGRIQS MYSVFHLMKE KGVLIDTVTY TSLIHWVSSS GDVDGAMRLW EEMRDNGCEP TVVSYTAYMK MLFADGRVEE ATEVYKEMLR SRVSPNCHTY TVLMEYLVAT GKCEEALDIF FKMQEIGVQP DKAACNILIA KALKFGETSF MTRVLVYMKE NGVVLRYPIF VEALETLKAA GESDDLLREV NSHISVESLC SSDIDETPTA EVNDTKNSDD SRVISSVLLM KQNLVAVDIL LNQMRDRNIK LDSFVVSAII ETNCDRCRTE GASLAFDYSL EMGIHLKKSA YLALIGNFLR SNELPKVIEV VKEMVKAQHS LGCYQGAMLI HRLGFGRRPR LAADVFDLLP DDQKGVAAYT ALMDVYISAG SPEKAMKILR EMREREIMPS LGTYDVLLSG LEKTSDFQKE VALLRKEKKS LVASARFREN VHVEDKICDL LFATNLL
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-

Multiple sequence alignmentEdit

A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.

Protein structureEdit

A predicted structure for this protein is available here.

ReferencesEdit

  1. This is a test reference.

Further readingEdit

General database linksEdit

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