In the plant Arabidopsis thaliana, At2g01190.1 is a gene model for the locus At2g01190.[1]
This gene model is classified by TAIR as "octicosapeptide/Phox/Bem1p (PB1) domain-containing protein". It is located on chr2, region 115022-117295 FORWARD.
Physiological function[]
At2g01190.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At2g01190.1/Expression
Subcellular localization[]
At2g01190.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At2g01190.1 has 720 amino-acid residues and a molecular weight Mw = 77.317 kDa.
The protein is relatively acidic; at the physiological pH 7.4, its net charge is -16.67, or -0.023 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At2g01190.1/Knockout
Protein interactions[]
At2g01190.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At2g01190.1 has 1 recognized domains:
- Domain_0089_0181 PB1 domain (PF00564, IPR000270) Description: Octicosapeptide/Phox/Bem1p_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At2g01190.1
MEPPPSSLSS
TAVASTSISA
ATAPVPPPLP
PHVTSSYPES
LDSSPRSRTT
DGWDDLPAPS
GGGGGGGGSA
VSSKLRFMCS
YGGHILPRPH
DKSLCYMGGD
TRIVVVDRNS
SLPSLIARLS
NTLLDGRSFT
LKYQLPSEDL
DSLISVTTDE
DLDNMIEEYD
RTISASNSTK
PSRLRLFLFT
SKPEATQSMG
QILESSAKSD
DWFLNALNSA
GLLNRGFSDS
DTNVNRLLGL
DDALALRSNS
GDNNNRDGDD
GSVKSAKQQQ
PPPPQQQEQQ
QGGQDVNCLP
DSPMLDTSSS
FGSTSSSPSL
ANLPPIRVHV
EEPGGVRTLP
DQRNLGIEEQ
FARFNVGNKH
QLHDDGFAAI
SSPPPMPVTI
ALPAAPVTAA
TVSNEFQARV
YSDDERSDHG
VQAGYRKPPT
PRSQPQNLPP
QQAHQLKSNS
GGGHELPSPN
SVSSDSSMSN
PMFHQRPSVY
QEPIAQIPSG
STVVTGMINP
SDPSTLLSQH
QNQDPAYILH
PQFEQQSAQS
QPQQQFIHTA
APPQYIHHHP
SSGLPVPTYI
QVYPSQQPQQ
SFHQHAGRLD
QQPYPVYYVT
APVPPRPYSM
PVPQSPSVSD
AAGSIPSNHP
NSTMMPPPPN
NHMRSVSSGK
PEMGQAGVYT
TAPGVGGAQM
VHQIPTNQQQ
FMGYSQIRHP
PQSGSAGNPN
YGYEYVDNAH
TQIYYTQPMG
HAQYQTMTGP
PPAMVMPDGS
AAAKLPAENM
TQQIRSSQPL
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.