At2g01180.1
From Proteins Wiki
In the plant Arabidopsis thaliana, At2g01180.1 is a gene model for the locus At2g01180.[1]
Also known as PAP1, LPP1, ATLPP1, and ATPAP1, this gene model is classified by TAIR as "ATPAP1 (PHOSPHATIDIC ACID PHOSPHATASE 1); phosphatidate phosphatase". It is located on chr2, region 107181-108554 REVERSE.
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[edit] Physiological function
At2g01180.1/Function NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
[edit] Tissue expression
[edit] Subcellular localization
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
[edit] Biophysical properties
The gene model At2g01180.1 has 327 amino-acid residues and a molecular weight Mw = 36.686 kDa.
The protein is relatively neutral; at the physiological pH 7.4, its net charge is 3.65, or 0.011 per residue. NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
[edit] Knockout phenotype
[edit] Protein interactions
At2g01180.1/Interactions This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
[edit] Domain structure
The gene model At2g01180.1 has 1 recognized domains:
- Domain_0124_0273 PAP2 domain (PF01569, IPR000326) Description: Phosphoesterase, PA-phosphatase related
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
[edit] Amino-acid sequence
>At2g01180.1
MTIGSFFSSL
LFWRNSQDQE
AQRGRMQEID
LSVHTIKSHG
GRVASKHKHD
WIILVILIAI
EIGLNLISPF
YRYVGKDMMT
DLKYPFKDNT
VPIWSVPVYA
VLLPIIVFVC
FYLKRTCVYD
LHHSILGLLF
AVLITGVITD
SIKVATGRPR
PNFYWRCFPD
GKELYDALGG
VVCHGKAAEV
KEGHKSFPSG
HTSWSFAGLT
FLSLYLSGKI
KAFNNEGHVA
KLCLVIFPLL
AACLVGISRV
DDYWHHWQDV
FAGALIGTLV
AAFCYRQFYP
NPYHEEGWGP
YAYFKAAQER
GVPVTSSQNG
DALRAMSLQM
DSTSLENMES
GTSTAPR
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
[edit] Multiple sequence alignment
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
[edit] Protein structure
A predicted structure for this protein is available here.
[edit] References
- ↑ This is a test reference.
