At2g01140.1
From Proteins Wiki
In the plant Arabidopsis thaliana, At2g01140.1 is a gene model for the locus At2g01140.[1]
This gene model is classified by TAIR as "fructose-bisphosphate aldolase, putative". It is located on chr2, region 95005-96490 REVERSE.
Contents |
[edit] Physiological function
At2g01140.1/Function NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
[edit] Tissue expression
[edit] Subcellular localization
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
[edit] Biophysical properties
The gene model At2g01140.1 has 391 amino-acid residues and a molecular weight Mw = 42.329 kDa.
The protein is relatively neutral; at the physiological pH 7.4, its net charge is 1.99, or 0.005 per residue. NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
[edit] Knockout phenotype
[edit] Protein interactions
At2g01140.1/Interactions This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
[edit] Domain structure
The gene model At2g01140.1 has 1 recognized domains:
- Domain_0047_0391 Glycolytic domain (PF00274, IPR000741) Description: Fructose-bisphosphate aldolase, class-I;Molecular Function: fructose-bisphosphate aldolase activity (GO:0004332), Biological Process: glycolysis (GO:0006096)
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
[edit] Amino-acid sequence
>At2g01140.1
MASASFVKPN
TLSSPWIGQR
SFAHTSASSS
PPPRVSFAIR
AGAYSDELVK
TAKSIASPGR
GILAIDESNA
TCGKRLASIG
LDNTEDNRQA
YRQLLLTTPG
LGDYISGSIL
FEETLYQSTK
DGKTFVDCLR
DANIVPGIKV
DKGLSPLAGS
NEESWCQGLD
GLASRSAEYY
KQGARFAKWR
TVVSVPCGPS
ALAVKEAAWG
LARYAAISQD
NGLVPIVEPE
ILLDGDHPIE
RTLEVAEKVW
SEVFFYLAQN
NVMFEGILLK
PSMVTPGAEH
KNKASPETVA
DFTLTMLKRR
VPPAVPGIMF
LSGGQSEAEA
TLNLNAMNQS
PNPWHVSFSY
ARALQNSVLR
TWQGKPEKIE
ASQKALLVRA
KANSLAQLGK
YSAEGENEDA
KKGMFVKGYT
Y
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
[edit] Multiple sequence alignment
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
[edit] Protein structure
A predicted structure for this protein is available here.
[edit] References
- ↑ This is a test reference.
