At2g01110.1

From Proteins Wiki

In the plant Arabidopsis thaliana, At2g01110.1 is a gene model for the locus At2g01110.^[1]

Also known as UNE3, PGA2, TATC, and APG2, this gene model is classified by TAIR as "APG2 (ALBINO AND PALE GREEN 2)". It is located on chr2, region 83786-85088 REVERSE.

Contents 1 Physiological function 2 Tissue expression 3 Subcellular localization 4 Biophysical properties 5 Knockout phenotype 6 Protein interactions 7 Domain structure 8 Amino-acid sequence 9 Multiple sequence alignment 10 Protein structure 11 References 12 Further reading 13 General database links

[edit] Physiological function

At2g01110.1/Function NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

[edit] Tissue expression

At2g01110.1/Expression

[edit] Subcellular localization

At2g01110.1/Localization

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

[edit] Biophysical properties

The gene model At2g01110.1 has 340 amino-acid residues and a molecular weight Mw = 37.362 kDa.

The protein is relatively acidic; at the physiological pH 7.4, its net charge is -5.12, or -0.015 per residue. NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

[edit] Knockout phenotype

At2g01110.1/Knockout

[edit] Protein interactions

At2g01110.1/Interactions This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

[edit] Domain structure

The gene model At2g01110.1 has 1 recognized domains:

• Domain_0122_0323 TatC domain (PF00902, IPR002033) Description: Sec-independent periplasmic protein translocase

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

[edit] Amino-acid sequence

>At2g01110.1
MSSTSTSSAL IHHFRLTTRN LGSPTKQRCP YAVTFCNSWR EAGLRYSVTQ RRSKGFGPVS ALNDDDSPTE TTPGVGSAVE DRPPDSSEDR SSSVYEFLYP RKEELPDDKE MTIFDHLEEL RERIFVSVLA VGAAILGCFA FSKDLIVFLE APVKTQGVRF LQLAPGEFFF TTLKVSGYCG LLLGSPVILY EIIAFVLPGL TRAERRFLGP IVFGSSLLFY AGLAFSYWVL TPAALNFFVN YAEGVVESLW SIDQYFEFVL VLMFSTGLSF QVPVIQLLLG QVGVVSGDQM LSIWRYVVVG AVVAAAVVTP STDPVTQMLL ATPLLGLYLG GAWMVKLTGR MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-

[edit] Multiple sequence alignment

A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.

[edit] Protein structure

A predicted structure for this protein is available here.

[edit] References

[edit] Further reading

• PubMed references for At2g01110

• PubMed Central references for At2g01110

• Google Scholar references for At2g01110

[edit] General database links