At1g65620.3
From Proteins Wiki
In the plant Arabidopsis thaliana, At1g65620.3 is a gene model for the locus At1g65620.[1]
Also known as AS2, this gene model is classified by TAIR as "AS2 (ASYMMETRIC LEAVES 2)". It is located on chr1, region 24403809-24404408 FORWARD.
Contents |
[edit] Physiological function
At1g65620.3/Function NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
[edit] Tissue expression
[edit] Subcellular localization
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
[edit] Biophysical properties
The gene model At1g65620.3 has 199 amino-acid residues and a molecular weight Mw = 21.709 kDa.
The protein is relatively neutral; at the physiological pH 7.4, its net charge is 0.21, or 0.001 per residue. NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
[edit] Knockout phenotype
[edit] Protein interactions
At1g65620.3/Interactions This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
[edit] Domain structure
The gene model At1g65620.3 has 1 recognized domains:
- Domain_0009_0109 DUF260 domain (PF03195, IPR004883) Description: Lateral organ boundaries, LOB
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
[edit] Amino-acid sequence
>At1g65620.3
MASSSTNSPC
AACKFLRRKC
QPECVFAPYF
PPDQPQKFAN
VHKVFGASNV
TKLLNELHPS
QREDAVNSLA
YEADMRLRDP
VYGCVGVISL
LQHQLRQLQI
DLSCAKSELS
KYQSLGILAA
THQSLGINLL
AGAADGTATA
VRDHYHHHQF
FPREQMFGGL
DVPAGNNYDG
GILAIGQITQ
FQQPRAAAGD
DGRRTVDPS
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
[edit] Multiple sequence alignment
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
[edit] Protein structure
A predicted structure for this protein is available here.
[edit] References
- ↑ This is a test reference.
