At1g63940.4

In the plant Arabidopsis thaliana, At1g63940.4 is a gene model for the locus At1g63940.^[1]

This gene model is classified by TAIR as "monodehydroascorbate reductase, putative". It is located on chr1, region 23733869-23737197 FORWARD.

Physiological function[]

At1g63940.4/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g63940.4/Expression

Subcellular localization[]

At1g63940.4/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g63940.4 has 482 amino-acid residues and a molecular weight Mw = 52.116 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 1.21, or 0.003 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g63940.4/Knockout

Protein interactions[]

At1g63940.4/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g63940.4 has 1 recognized domains:

Domain_0056_0350 Pyr_redox_2 domain (PF07992, IPR013027) Description: FAD-dependent pyridine nucleotide-disulphide oxidoreductase_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g63940.4 MALASTTLPT KSGLSLWCPS SPSLARRFPA RFSPIGSRIA SRSLVTASFA NENREFVIVG GGNAAGYAAR TFVENGMADG RLCIVTKEAY APYERPALTK AYLFPPEKKP ARLPGFHTCV GGGGERQTPD WYKEKGIEVI YEDPVAGADF EKQTLTTDAG KQLKYGSLII ATGCTASRFP DKIGGHLPGV HYIREVADAD SLIASLGKAK KIVIVGGGYI GMEVAAAAVA WNLDTTIVFP EDQLLQRLFT PSLAQKYEEL YRQNGVKFVK GASINNLEAG SDGRVSAVKL ADGSTIEADT VVIGIGAKPA IGPFETLAMN KSIGGIQVDG LFRTSTPGIF AIGDVAAFPL KIYDRMTRVE HVDHARRSAQ HCVKSLLTAH TDTYDYLPYF YSRVFEYEGS PRKVWWQFFG DNVEVGNFDP KIATFWIESG RLKGVLVESG SPEEFQLLPK LARSQPLVDK AKLASASSVE EALEIAQAAL QS ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-