At1g55460.1

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In the plant Arabidopsis thaliana, At1g55460.1 is a gene model for the locus At1g55460.^[1]

[edit] Physiological function

At1g55460.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

[edit] Tissue expression

At1g55460.1/Expression

[edit] Subcellular localization

At1g55460.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

[edit] Biophysical properties

At1g55460.1/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

[edit] Knockout phenotype

At1g55460.1/Knockout

[edit] Protein interactions

At1g55460.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

[edit] Domain structure

At1g55460.1/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

[edit] Amino-acid sequence

>At1g55460.1 MGKNDFLTPK AIANRIKAKG LQKLRWYCQM CQKQCRDENG FKCHCMSESH QRQMQVFGQN PTRVVDGYSE EFEQTFLDLM RRSHRFSRIA ATVVYNEYIN DRHHVHMNST EWATLTEFIK HLGKTGKCKV EETPKGWFIT YIDRDSETLF KERLKNKRVK SDLAEEEKQE REIQRQIERA AEKLNGGGGE GETSGNDEVV DDGDDERKKD EDLRLKSGVK VGFALGGGVK QVATGKERGE SSKLLFGDEE NDKVERGEKR KRSGDSGRSE KERRSALDEL MKEEEKKKER MNRKDYWLFE GIIVKVMSKA LAEKGYYKQK GVVKKVIDNY VGEIKMLDSK HVLRVDQKEL ETVLPQIGGM VKIVNGAYRG SNARLLGVDT EKFCAKVQIE KGVYDGRVIK SIEYEDICKL A ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-