In the plant Arabidopsis thaliana, At1g54780.1 is a gene model for the locus At1g54780.[1]
This gene model is classified by TAIR as "thylakoid lumen 18.3 kDa protein". It is located on chr1, region 20443200-20444620 FORWARD.
Physiological function[]
At1g54780.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g54780.1/Expression
Subcellular localization[]
At1g54780.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At1g54780.1 has 285 amino-acid residues and a molecular weight Mw = 31.141 kDa.
The protein is relatively neutral; at the physiological pH 7.4, its net charge is 3.33, or 0.012 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g54780.1/Knockout
Protein interactions[]
At1g54780.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At1g54780.1 has 1 recognized domains:
- Domain_0105_0229 DUF477 domain (PF04536, IPR007621) Description: Protein of unknown function DUF477_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g54780.1
METLLSPRAL
SPPLNPKPLS
LHQTKPTSHS
LSLSKPTTFS
GPKHLSTRFT
KPESRNWLID
AKQGLAALAL
SLTLTFSPVG
TALASEFNIL
NDGPPKETYV
VDDAGVLSRV
TKSDLKKLLS
DLEYRKKLRL
NFITVRKLTS
KADAFEYADQ
VLEKWYPSIE
EGNNKGIVVL
ITSQKEGAIT
GGPAFIEAVG
ENILDATVSE
NLPVLATDEK
YNEAVYSSAK
RLVAAIDGQP
DPGGPTVKDS
KRESNFKTKE
ETDEKRGQFS
LVVGGLLVIA
FVVPMAQYFA
YVSRK
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.