At1g51680.3

From Proteins Wiki

In the plant Arabidopsis thaliana, At1g51680.3 is a gene model for the locus At1g51680.^[1]

At1g51680.3/Header

Contents 1 Physiological function 2 Tissue expression 3 Subcellular localization 4 Biophysical properties 5 Knockout phenotype 6 Protein interactions 7 Domain structure 8 Amino-acid sequence 9 Multiple sequence alignment 10 Protein structure 11 References 12 Further reading 13 General database links

[edit] Physiological function

At1g51680.3/Function NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

[edit] Tissue expression

At1g51680.3/Expression

[edit] Subcellular localization

At1g51680.3/Localization

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

[edit] Biophysical properties

At1g51680.3/Biophysics NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

[edit] Knockout phenotype

At1g51680.3/Knockout

[edit] Protein interactions

At1g51680.3/Interactions This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

[edit] Domain structure

At1g51680.3/Domains NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

[edit] Amino-acid sequence

>At1g51680.3
MAPQEQAVSQ VMEKQSNNNN SDVIFRSKLP DIYIPNHLSL HDYIFQNISE FATKPCLING PTGHVYTYSD VHVISRQIAA NFHKLGVNQN DVVMLLLPNC PEFVLSFLAA SFRGATATAA NPFFTPAEIA KQAKASNTKL IITEARYVDK IKPLQNDDGV VIVCIDDNES VPIPEGCLRF TELTQSTTEA SEVIDSVEIS PDDVVALPYS SGTTGLPKGV MLTHKGLVTS VAQQVDGENP NLYFHSDDVI LCVLPMFHIY ALNSIMLCGL RVGAAILIMP KFEINLLLEL IQRCKVTVAP MVPPIVLAIA KSSETEKYDL SSIRVVKSGA APLGKELEDA VNAKFPNAKL GQGYGMTEAG PVLAMSLGFA KEPFPVKSGA CGTVVRNAEM KIVDPDTGDS LSRNQPGEIC IRGHQIMKGY LNNPAATAET IDKDGWLHTG DIGLIDDDDE LFIVDRLKEL IKYKGFQVAP AELEALLIGH PDITDVAVVA MKEEAAGEVP VAFVVKSKDS ELSEDDVKQF VSKQVKSCVL QENQQSVLH MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-

[edit] Multiple sequence alignment

A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.

[edit] Protein structure

A predicted structure for this protein is available here.

[edit] References

[edit] Further reading

• PubMed references for At1g51680

• PubMed Central references for At1g51680

• Google Scholar references for At1g51680

[edit] General database links