At1g48650.1

In the plant Arabidopsis thaliana, At1g48650.1 is a gene model for the locus At1g48650.^[1]

This gene model is classified by TAIR as "helicase domain-containing protein". It is located on chr1, region 17993338-17998837 REVERSE.

Physiological function[]

At1g48650.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g48650.1/Expression

Subcellular localization[]

At1g48650.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g48650.1 has 1197 amino-acid residues and a molecular weight Mw = 134.278 kDa.

The protein is relatively basic; at the physiological pH 7.4, its net charge is 26.53, or 0.022 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g48650.1/Knockout

Protein interactions[]

At1g48650.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g48650.1 has 5 recognized domains:

Domain_0302_0463 DEAD domain (PF00270, IPR011545) Description: DEAD/DEAH box helicase, N-terminal;Molecular Function: nucleic acid binding (GO:0003676), Molecular Function: helicase activity (GO:0004386), Molecular Function: ATP binding (GO:0005524)

Domain_0604_0698 Helicase_C domain (PF00271, IPR001650) Description: Helicase, C-terminal;Molecular Function: nucleic acid binding (GO:0003676), Molecular Function: helicase activity (GO:0004386), Molecular Function: ATP binding (GO:0005524)

Domain_0760_0851 HA2 domain (PF04408, IPR007502) Description: Helicase-associated region;Molecular Function: helicase activity (GO:0004386)

Domain_0888_0998 DUF1605 domain (PF07717, IPR011709) Description: Protein of unknown function DUF1605;Molecular Function: ATP binding (GO:0005524), Molecular Function: ATP-dependent helicase activity (GO:0008026)

Domain_1101_1163 dsrm domain (PF00035, IPR001159) Description: Double-stranded RNA binding;Molecular Function: double-stranded RNA binding (GO:0003725), Cellular Component: intracellular (GO:0005622)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g48650.1 MQLYSAIFRG HIRPNAVVGA ASMFIQHNSI QLHRSPKLLL RPSSVVRSLH CRRSGGLVTH SQRSRVLCVK AARGDASSST LGIEWRAANL PYFQRQNSGY GRIAYNDYES SDESDRDVGS SQSQQMAGST LDNIDQWRFK LTMLLRNKED QEVVSRERKD RRDFDHISAL ATRMGLHSRQ YSKIVVISKA PLPNYRPDLD DKRPQREVVL PFGLQSEVDA HLHSFLDQKK TLIPEMPRQN SSESLANGYG NYETPETVMQ NSLARERILR PRSLQLKSKQ QQWVDSPEGQ KMVGFRKTLP AYKEKDALLK AIAANQVVVV SGETGCGKTT QLPQYILESE IEAARGATCS IICTQPRRIS AISVSERVAA ERGEQIGESV GYKVRLEGMR GRDTRLLFCT TGVLLRRLLV DRSLKGVTHV VVDEIHERGM NEDFLLIVLK DLLPRRPDLK LILMSATLNA ELFSSYFGGA PAMHIPGFTY PVRAHFLEDY LETSGYRLTT YNQIDDYGEE KTWKMQKQAQ FKKRKSLISS AVEDALEAAD FKGYNFRTRD SLSCWSPDSI GFNLIENVLC HIVKGERPGA VLVFMTGWDD INSLKNQLEA HSLLGDPNKV LLLACHGSMA SSEQRLIFDR PPEGIRKIVL ATNMAETSIT INDVVYVIDC GKAKETSYDA LNNTPCLLPS WISKAAARQR RGRAGRVMPG ECYHLYPRCV YEAFADYQQP ELLRTPLQSL CLQIKSLGLG SISEFLSRAL QPPEALSVQN AVEYLKIIGA LDDDENLTPL GKNLSMLPVE PKLGKMLILG AIFNCLDPVM TVVAGLSVRD PFLMPFDKKD LAETARSKFS GRDYSDHLTL VRAYNGWKDA ERTHSGYDYC WKNFLSSQTL KAMDSMRKQF FNLLKEASLI DNIEGCSKLS HDEHLVRAII CAGMFPGVCS VVNKEKSITL KTMEDGQVLL YSSSVNGNVP MIPFPWLVFN DKVKVNSVFL RDSTAVSDSV LLLFGDKISS GGFDGHLKML GGYLEFFMKP TLAYTYLSLK RELDELIQNK LVNPKLDIQL YDKLMTAIRL LVSEDQCEGR FVYGRKALSP TPAKKLKDVG AQLQNSGGEN NKNQLQTLLA RAGHGSPVYK TRQLKNNQFR SMVTFNGLDF MGKPCGSKKN AEKDAAHEAL LWLQGESKSS LNDLNHMSML LKKNKSKNHA KASTKWG ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-