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At1g47890.1

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In the plant Arabidopsis thaliana, At1g47890.1 is a gene model for the locus At1g47890.[1]

At1g47890.1/Header

Physiological functionEdit

At1g47890.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expressionEdit

At1g47890.1/Expression

Subcellular localizationEdit

At1g47890.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical propertiesEdit

At1g47890.1/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotypeEdit

At1g47890.1/Knockout

Protein interactionsEdit

At1g47890.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structureEdit

At1g47890.1/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequenceEdit

>At1g47890.1
MELYVFFIYV VGKTLHKKIQ NTNLLLLFGK LCFFTNIKVF IFSEKMSFLI RSICFLILIP SFLITFVSAT QHLCHSDQKD ALLDFKNEFG MVDSKSWVNK SDCCSWDGIT CDAKSGNVIG LDLSSIFLYG QLKSNSSLFK LRHLRDLNLA NNNFNNSPIP AEFDKLTGLE RLDLSQSSLS GQIPINLLQL TKLVSLDLSS SDFFGDESFH YLSIDKSFLP LLARNLRNLR ELDMSYVKIS SEIPEEFSNI RSLRSLNLNG CNLFGEFPSS ILLIPNLQSI DLGNNPNLRG NLPVFHENNS LLKLTILYTS FSGAIPDSIS SLKNLTSLTL SVSYFSGKIP FSLGNLSHLS HLSLSSNNLI GEIPSSIGNL NQLTNFYVGG NKLSGNLPAT LSNLTKLNTI SLSSNQFTGS LPPSISQLSK LKFFFADDNP FIGAILSPLL KIPSLTRIHL SYNQLNDLVG IENIFMLPNL ETFYIYHYNY TKVRPLDLNV FSSLKQLGTL YISRIPISTT NITSDFPSNL EYLSLRSCNI TDFPEFIRKG RNLQILDLSN NKIKGQVPDW LWRMPTLNSV DLSNNSLSGF HVSVKASPES QLTSVDLSSN AFQGPLFLPS KSLRYFSGSN NNFTGKIPRS ICGLSSLEIL DLSNNNLNGS LPWCLETLMS SLSDLDLRNN SLSGSLPEIF MNATKLRSLD VSHNRMEGKL PGSLTGCSSL EVLNVGSNRI NDMFPFELNS LQKLQVLVLH SNKFHGTLHN VDGVWFGFPQ LQIIDVSHND FFGILPSDYF MNWTAMSSKK DNNIEPEYIQ NPSVYGSSLG YYTSLVLMSK GVSMEMERVL TIYTAIDLSG NQLHGKIPDS IGLLKELRIL NMSSNGFTGH IPSSLANLKN LESLDISQNN ISGEIPPELG TLSSLAWINV SHNQLVGSIP QGTQFQRQKC SSYEGNPGLN GPSLENVCGH IKESTPTQTE PLETKEEEEE ESFSWIAAGL GFAPGVVFGL AMGYIVVSYK HQWFMKTFGR SKQQNTRTR
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-

Multiple sequence alignmentEdit

A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.

Protein structureEdit

A predicted structure for this protein is available here.

ReferencesEdit

  1. This is a test reference.

Further readingEdit

General database linksEdit

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