At1g24590.1

In the plant Arabidopsis thaliana, At1g24590.1 is a gene model for the locus At1g24590.^[1]

Also known as DRN-LIKE, SOB2, ESR2, and DRNL, this gene model is classified by TAIR as "DRNL (DORNROSCHEN-LIKE); DNA binding / transcription factor". It is located on chr1, region 8714375-8715295 REVERSE.

Physiological function[]

At1g24590.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g24590.1/Expression

Subcellular localization[]

At1g24590.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g24590.1 has 306 amino-acid residues and a molecular weight Mw = 33.825 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is -2.45, or -0.008 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g24590.1/Knockout

Protein interactions[]

At1g24590.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g24590.1 has 1 recognized domains:

Domain_0056_0119 AP2 domain (PF00847, IPR001471) Description: Pathogenesis-related transcriptional factor and ERF;Molecular Function: transcription factor activity (GO:0003700), Cellular Component: nucleus (GO:0005634), Biological Process: regulation of transcription, DNA-dependent (GO:0006355)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g24590.1 MEEAIMRLEG AEHRETNIHS LKRKPSRTSS TAPGSPGGVT TAKAASGAGA SGVSTIRYRG VRRRPWGRYA AEIRDPLSKE RRWLGTFDTA EEAACAYDCA ARAMRGLKAR TNFVYPMPSL DSYHHRIFSS PPMNMFLLRD VLNSQSLSPL TTFAYPPCNL SNVNDVVHES FTNVNDVCED LSPKAKRSST IENESLISNI FEPEPASSGL LQEIVQGFLP KPISQHASIP PKSNQQSVGV FPTMPESGFQ TDVRLADFHV EGNGFGQVKY HGELGWADHE NGFDSAKMQQ NGNGGMFYQY CFHDDY ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-