At1g15980.1
From Proteins Wiki
In the plant Arabidopsis thaliana, At1g15980.1 is a gene model for the locus At1g15980.[1]
This gene model is classified by TAIR as "similar to Os08g0276100 [Oryza sativa (japonica cultivar-group)] (GB:NP_001061432.1); similar to COG0859: ADP-heptose:LPS heptosyltransferase [Nostoc punctiforme PCC 73102] (GB:ZP_00111141.1); contains domain UDP-Glycosyltransferase/glycogen phosphorylase (SSF53756)". It is located on chr1, region 5489308-5491193 FORWARD.
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[edit] Physiological function
At1g15980.1/Function NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
[edit] Tissue expression
[edit] Subcellular localization
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
[edit] Biophysical properties
The gene model At1g15980.1 has 461 amino-acid residues and a molecular weight Mw = 51.021 kDa.
The protein is relatively neutral; at the physiological pH 7.4, its net charge is -1.13, or -0.002 per residue. NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
[edit] Knockout phenotype
[edit] Protein interactions
At1g15980.1/Interactions This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
[edit] Domain structure
The gene model At1g15980.1 has no known domains. NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
[edit] Amino-acid sequence
>At1g15980.1
MASSLPLLPK
PISPFFKTPP
FSTSKPLVFL
NFQTRLTSRS
SDVSVNLKKK
NNPWLDPFDS
GEDPDNEYGS
LFADGKQDED
PRPPDNPDNP
YGFLKFPKGY
TVELASLPLK
IRGDVRRCCC
VISGGVYENL
LFFPTIQLIK
DRYPGVQVDI
LTTERGKQTY
ELNKNVRWAN
VYDPDDHWPE
PAEYTDMIGL
LKGRYYDMVL
STKLAGLGHA
AFLFMTTARD
RVSYIYPNVN
SAGAGLMLSE
TFTAENTNLS
ELGYSMYTQM
EDWLGRPFRS
VPRTPLLPLR
VSISRKVKEV
VAAKYRNAGA
VTGKFIVIHG
IESDSKASMQ
SKGDADSLLS
LEKWAKIIKG
VRGFKPVFVI
PHEKERENVE
DFVGDDTSIV
FITTPGQLAA
LINDSAGVIA
TNTAAIQLAN
ARDKPCIGLF
SSEEKGKLFV
PYAEEKSNCV
IIASKTGKLA
DIDIGTVKNA
MQVFEGSLAL
V
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
[edit] Multiple sequence alignment
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
[edit] Protein structure
A predicted structure for this protein is available here.
[edit] References
- ↑ This is a test reference.
