At1g12240.1

In the plant Arabidopsis thaliana, At1g12240.1 is a gene model for the locus At1g12240.^[1]

At1g12240.1/Header

Physiological function[]

At1g12240.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g12240.1/Expression

Subcellular localization[]

At1g12240.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

At1g12240.1/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g12240.1/Knockout

Protein interactions[]

At1g12240.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

At1g12240.1/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g12240.1 MASSDALLPI SAREEEPLCP YTRLPMADPN QETHGPRRRR PFKGLLAVSF GLLFIAFYVA LIATHDGSRS NDEGIDETET ITSRARLAGV SEKRNDGLWK LSGDRNTPAF EWNNSMLSWQ RTAFHFQPEQ NWMNDPNGPL FYKGWYHFFY QYNPNAAVWG DIVWGHAVSR DLIHWVHLPI AMVADQWYDS NGVWTGSATF LPDGSIVMLY TGSTDKAVQV QNLAYPEDPN DPLLLKWVKF PGNPVLVPPP GILPKDFRDP TTAWKTSEGK WRITIGSKLN KTGISLVYDT IDFKTYEKLD TLLHRVPNTG MWECVDFYPV SKTAGNGLDT SVNGPDVKHI VKASMDDTRF DHYAVGTYFD SNGTWIPDDP TIDVGMTASL RYDYGKFYAS KSFYDQNKGR RVLWSWIGES DSEASDVQKG WSSLQGIPRT VVLDTKTGKN LVQWPVEEIK SLRLSSKQFD LEVGPGSVVP VDVGSAAQLD IEAEFEINKE SLDKIIGNAS VVAEAEEFSC EKSGGSTVRG ALGPFGFSVL ATESLSEQTP VYFYVAKGKD SELKTFFCTD TSRSSVANDV VKPIYGSVVP VLKGEKLTMR ILVDHSIVEA FGQGGRTCIT SRVYPTTAIY GAAKLFLFNN ALDATVTASF TVWQMNSAFI HPYSDEAVRA LSRT ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-