At1g12130.1

In the plant Arabidopsis thaliana, At1g12130.1 is a gene model for the locus At1g12130.^[1]

This gene model is classified by TAIR as "flavin-containing monooxygenase family protein / FMO family protein". It is located on chr1, region 4118592-4120869 FORWARD.

Physiological function[]

At1g12130.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g12130.1/Expression

Subcellular localization[]

At1g12130.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g12130.1 has 470 amino-acid residues and a molecular weight Mw = 53.824 kDa.

The protein is relatively acidic; at the physiological pH 7.4, its net charge is -11.33, or -0.024 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g12130.1/Knockout

Protein interactions[]

At1g12130.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g12130.1 has 2 recognized domains:

Domain_0012_0045 Pyr_redox domain (PF00070, IPR001327) Description: Pyridine nucleotide-disulphide oxidoreductase, NAD-binding region;Biological Process: electron transport (GO:0006118), Molecular Function: disulfide oxidoreductase activity (GO:0015036)

Domain_0012_0047 Pyr_redox_2 domain (PF07992, IPR013027) Description: FAD-dependent pyridine nucleotide-disulphide oxidoreductase_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g12130.1 MTPPPNSISS RNVAVIGAGA AGLVAARELR REGHTVTIFE RQKQVGGLWV CTPNVEPDLL SIDPDRTVVH SSVYQSLRTN LPRECMGYSD FPFVTRPDDE SRDPRRYPDH REVMRYLQDF AKEFKIEEMI RFETEVFRVE PTAENSCKWR VQFRSSSGVS GEDIFDAVVI CNGHFTEPRL AHIPGIESWP GKQIHSHNYR VSDPFKGQVV IVIGYQSSGS DISRDIAILA KEVHIAAKSD AYAKESSIYS NLHFHPTIDR VYEDGSVVFQ DGKLIFADAI VHCTGYKYCF PFLETKGYVN VEDNRVGPLY KHVFPPALAP GLSFIGLPSM ALQFFMFEIQ SRWVASVLSG RVKLPSEEQM MEDVIAFYAK LKSLGIPKRF THFLTDPQWT PMFEKLKPHE AVLISQSEYF NWIAEQCGCS SIERWREEQY NIAIKKDDDN FRDEWDDDDQ IIQEAYRDFA KFKPSKVWST ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-