At1g10510.1
From Proteins Wiki
In the plant Arabidopsis thaliana, At1g10510.1 is a gene model for the locus At1g10510.[1]
Also known as EMB2004, this gene model is classified by TAIR as "EMB2004 (EMBRYO DEFECTIVE 2004); protein binding". It is located on chr1, region 3461772-3465591 FORWARD.
Contents |
[edit] Physiological function
At1g10510.1/Function NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
[edit] Tissue expression
[edit] Subcellular localization
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
[edit] Biophysical properties
The gene model At1g10510.1 has 605 amino-acid residues and a molecular weight Mw = 64.722 kDa.
The protein is relatively neutral; at the physiological pH 7.4, its net charge is 0.78, or 0.001 per residue. NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
[edit] Knockout phenotype
[edit] Protein interactions
At1g10510.1/Interactions This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
[edit] Domain structure
The gene model At1g10510.1 has 1 recognized domains:
- Domain_0339_0365 LRR_1 domain (PF00560, IPR001611) Description: Leucine-rich repeat
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
[edit] Amino-acid sequence
>At1g10510.1
MASSSTSSLN
LHSLPKASSG
LGQWKSGFRY
ELLGSSVSRN
RLFVSPVVIH
HRSPRLPAIK
AAYNSDGGSK
RSRVYKESQA
ASGFPNAKVQ
QIASSVLPLG
SFAVVTFVLW
KVVGKFMSPK
SPKTSAGENN
SSTQGVKWSI
GAGTNLLQGF
AAKVDREAKQ
RLNEFAKELR
SFRSVDMSGC
NFGDEGLFFL
AESLGYNQTV
EEVSFSANGI
TAAGVKAFDG
VLQSNIMLKI
LNLSGNPIGD
EGAKTLCATL
MENSSIEILQ
LNSTDIGDEG
AKEIAELLKR
NSTLRIIELN
NNMIDYSGFT
SLAGALLENN
TIRNLHLNGN
YGGALGANAL
AKGLEGNKSL
RELHLHGNSI
GDEGTRALMA
GLSSHKGKVA
LLDLGNNSIS
AKGAFYVAEY
IKRSKSLVWL
NLYMNDIGDE
GAEKIADSLK
QNRSIATIDL
GGNNIHAEGV
NAIAQALKDN
AIITTLEVGY
NPIGPDGAKA
LSEILKFHGN
VKTLKLGWCQ
IAAKGAEHVA
DMLRYNNTIS
VLDLRANGLR
DEGASCLARS
LKVVNEALTS
VDLGFNEIRD
DGAFAIAQAL
KANEDVTVTS
INLGNNFITK
FGQSALTDAR
DHVLEMTEKE
VEIFF
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
[edit] Multiple sequence alignment
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
[edit] Protein structure
A predicted structure for this protein is available here.
[edit] References
- ↑ This is a test reference.
