At1g07320.2

In the plant Arabidopsis thaliana, At1g07320.2 is a gene model for the locus At1g07320.^[1]

Also known as RPL4, this gene model is classified by TAIR as "RPL4 (ribosomal protein L4); structural constituent of ribosome". It is located on chr1, region 2249189-2250188 FORWARD.

Physiological function[]

At1g07320.2/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g07320.2/Expression

Subcellular localization[]

At1g07320.2/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g07320.2 has 280 amino-acid residues and a molecular weight Mw = 30.417 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 3.34, or 0.012 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g07320.2/Knockout

Protein interactions[]

At1g07320.2/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g07320.2 has 1 recognized domains:

Domain_0066_0260 Ribosomal_L4 domain (PF00573, IPR002136) Description: Ribosomal protein L4/L1e;Molecular Function: structural constituent of ribosome (GO:0003735), Cellular Component: intracellular (GO:0005622), Cellular Component: ribosome (GO:0005840), Biological Process: protein biosynthesis (GO:0006412)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g07320.2 MASSATAPNS LSFFSSSLFL SSSHQIPKTY ISVSKLGSGR VSKPLSVSSQ LATLPILSFE GEKVGETYLD LKAAPEDTAR AVVHRAIVTD LNNKRRGTAS TLTRGEVRGG GIKPYSQKKT GHARRGSQRT PLRPGGGVVF GPRPKDWSIK INRKEKKLAI STALSSAASA EGGAIVVEEF GEKFEKPKTK DFLAAMQRWG LDPKEKAMFL MIDVDENVAK SSRNIGTLRM LTPRTLNLFD ILNADKLVLT PAAVEFLNAR YGVDAVEEED DDEDETEEEA ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-