In the plant Arabidopsis thaliana, At1g05800.1 is a gene model for the locus At1g05800.[1]
This gene model is classified by TAIR as "lipase class 3 family protein". It is located on chr1, region 1741203-1742618 FORWARD.
Physiological function[]
At1g05800.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g05800.1/Expression
Subcellular localization[]
At1g05800.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At1g05800.1 has 471 amino-acid residues and a molecular weight Mw = 52.808 kDa.
The protein is relatively basic; at the physiological pH 7.4, its net charge is 9.43, or 0.020 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g05800.1/Knockout
Protein interactions[]
At1g05800.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At1g05800.1 has 1 recognized domains:
- Domain_0200_0359 Lipase_3 domain (PF01764, IPR002921) Description: Lipase, class 3;Molecular Function: triacylglycerol lipase activity (GO:0004806), Biological Process: lipid metabolism (GO:0006629)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g05800.1
MAAKVFTQNP
IYSQSLVRDK
TPQQKHNLDH
FSISQHTSKR
LVVSSSTMSP
PISSSPLSLP
SSSSSQAIPP
SRAPAVTLPL
SRVWREIQGS
NNWENLIEPL
SPILQQEITR
YGNLLSASYK
GFDLNPNSKR
YLSCKYGKKN
LLKESGIHDP
DGYQVTKYIY
ATPDINLNPI
KNEPNRARWI
GYVAVSSDES
VKRLGRRDIL
VTFRGTVTNH
EWLANLKSSL
TPARLDPHNP
RPDVKVESGF
LGLYTSGESE
SKFGLESCRE
QLLSEISRLM
NKHKGEEISI
TLAGHSMGSS
LAQLLAYDIA
ELGMNQRRDE
KPVPVTVFSF
AGPRVGNLGF
KKRCEELGVK
VLRITNVNDP
ITKLPGFLFN
ENFRSLGGVY
ELPWSCSCYT
HVGVELTLDF
FDVQNISCVH
DLETYITLVN
RPRCSKLAVN
EDNFGGEFLN
RTSELMFSKG
RRQALHFTNA
ATNAAYLLCS
ISNHMLYYNI
F
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.