Wikia

In the plant Arabidopsis thaliana, At1g05420.1 is a gene model for the locus At1g05420.^[1]

Also known as ATOFP12, and OFP12, this gene model is classified by TAIR as "ATOFP12/OFP12 (Arabidopsis thaliana ovate family protein 12)". It is located on chr1, region 1590072-1590752 FORWARD.

Physiological functionEdit

At1g05420.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expressionEdit

At1g05420.1/Expression

Subcellular localizationEdit

At1g05420.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical propertiesEdit

The gene model At1g05420.1 has 226 amino-acid residues and a molecular weight Mw = 24.960 kDa.

The protein is relatively acidic; at the physiological pH 7.4, its net charge is -6.56, or -0.029 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotypeEdit

At1g05420.1/Knockout

Protein interactionsEdit

At1g05420.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structureEdit

The gene model At1g05420.1 has 1 recognized domains:

Domain_0158_0221 DUF623 domain (PF04844, IPR006458) Description: Protein of unknown function DUF623, plant_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequenceEdit

>At1g05420.1 MPRVMWKNFH LCFPSNLTKP SSSPSGATSD DPNRPSILLI NNFNLLYDDS SAAHRRLSKP LIHDVEPSST FTASTSTAAN SSSSSASYDD SDNYGFAPDD DSPPPDLTAV LASRRFFFSS PGCSNSITDS PDLRCRDNYD TATRLLTGGT AVKHYVQSPD PYNDFRRSMQ EMIDAVTNAG DLRRYEFLHE LLLSYLSLNA ADTHKFIIRA FADILVSLLS DGHRIS ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-