In the plant Arabidopsis thaliana, At1g03457.2 is a gene model for the locus At1g03457.[1]
At1g03457.2/Header
Physiological function[]
At1g03457.2/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g03457.2/Expression
Subcellular localization[]
At1g03457.2/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
At1g03457.2/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g03457.2/Knockout
Protein interactions[]
At1g03457.2/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
At1g03457.2/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g03457.2
MAEETMENEE
RVKLFVGQVP
KHMTEIQLLT
LFREFSIVNE
VNIIKEKTTR
APRGCCFLTC
PTREDADKVI
NSFHNKKTLP
GASSPLQVKY
ADGELERLDV
LDCSCNPEHK
LFVGMLPKNV
SETEVQSLFS
EYGTIKDLQI
LRGSLQTSKG
CLFLKYESKE
QAVAAMEALN
GRHIMEGANV
PLIVKWADTE
KERQARRLLK
VQSHVSRLDP
QNPSMFGALP
MSYVPPYNGY
GYHVPGTYGY
MLPPIQTQHA
FHNVISPNQG
NGRALQGTAL
TESVPPRLAP
RRNFPTALGN
YGYHGLQYPM
AFPRGMIPPR
LPLTTVSPGI
SNNGTSIPSS
LQTEGPAGAN
LFIYNIPREF
EDQELAATFQ
PFGKVLSAKV
FVDKATGISK
CFGFISYDSQ
AAAQNAINTM
NGCQLSGKKL
KVQLKRDNGQ
QQQQQQSKNP
LFNGLLNS
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.