In the plant Arabidopsis thaliana, At1g02110.1 is a gene model for the locus At1g02110.[1]
At1g02110.1/Header
Physiological function[]
At1g02110.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g02110.1/Expression
Subcellular localization[]
At1g02110.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
At1g02110.1/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g02110.1/Knockout
Protein interactions[]
At1g02110.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
At1g02110.1/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g02110.1
MGCTASKLDS
EDAVRRCKER
RRLMKDAVYA
RHHLAAAHSD
YCRSLRLTGS
ALSSFAAGEP
LSVSENTPAV
FLRPSSSQDA
PRVPSSHSPE
PPPPPIRSKP
KPTRPRRLPH
ILSDSSPSSS
PATSFYPTAH
QNSTYSRSPS
QASSVWNWEN
FYPPSPPDSE
YFERKARQNH
KHRPPSDYDA
ETERSDHDYC
HSRRDAAEEV
HCSEWGDDHD
RFTATSSSDG
DGEVETHVSR
SGIEEEPVKQ
PHQDPNGKEH
SDHVTTSSDC
YKTKLVVRHK
NLKEILDAVQ
DYFDKAASAG
DQVSAMLEIG
RAELDRSFSK
LRKTVYHSSS
VFSNLSASWT
SKPPLAVKYK
LDASTLNDEQ
GGLKSLCSTL
DRLLAWEKKL
YEDVKAREGV
KIEHEKKLSA
LQSQEYKGEA
VLTTSNAILR
LRDTDLVPQL
VELCHGLMYM
WKSMHEYHEI
QNNIVQQVRG
LINQTERGES
TSEVHRQVTR
DLESAVSLWH
SSFCRIIKFQ
REFICSLHAW
FKLSLVPLSN
GDPKKQRPDS
FALCEEWKQS
LERVPDTVAS
EAIKSFVNVV
HVISIKQAEE
VKMKKRTESA
GKELEKKASS
LRSIERKYYQ
AYSTVGIGPG
PEVLDSRDPL
SEKKCELAAC
QRQVEDEVMR
HVKAVEVTRA
MTLNNLQTGL
PNVFQALTSF
SSLFTESLQT
VCSRSYSIN
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.