In the plant Arabidopsis thaliana, At1g01550.1 is a gene model for the locus At1g01550.[1]
Also known as BPS1, this gene model is classified by TAIR as "BPS1 (BYPASS 1)". It is located on chr1, region 200526-201575 FORWARD.
Physiological function[]
At1g01550.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g01550.1/Expression
Subcellular localization[]
At1g01550.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At1g01550.1 has 349 amino-acid residues and a molecular weight Mw = 38.589 kDa.
The protein is relatively basic; at the physiological pH 7.4, its net charge is 5.99, or 0.017 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g01550.1/Knockout
Protein interactions[]
At1g01550.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At1g01550.1 has no known domains._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g01550.1
MARPQDPPRG
FFPFGNPFKN
LSSKNSVLSS
KLLPLLNNFE
TNLASSISKL
VPKEKSDILT
VSWMKQAMES
LCETHNGIKT
LITDLELPVS
DWEDKWVDVY
LDISVKLLDL
CNAFSSELTR
LNQGHLLLQF
ALHNLEANSP
QNLSKAQSSL
DSWKQHIVSK
NPRIENCRAI
LSSLVQTLNL
PKVKNSAKGK
VLMRALYGVK
VKTLYISGVF
AAAFSGSSQN
LMYLTVSNEL
PWAQSFMEVQ
NTMNAEIKNI
FLSDGLTVLK
ELEAVASGVK
KLYPAIQQGS
IDPISLQPLK
DSVTELSNGI
DLVSKEVDCF
FKILLSGRDT
LLENLRSMGA
STLQATSPKK
AAGKNYRGF
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.