In the plant Arabidopsis thaliana, At1g01440.1 is a gene model for the locus At1g01440.[1]
At1g01440.1/Header
Physiological function[]
At1g01440.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g01440.1/Expression
Subcellular localization[]
At1g01440.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
At1g01440.1/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g01440.1/Knockout
Protein interactions[]
At1g01440.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
At1g01440.1/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g01440.1
MRTSGDVYGA
DTGSSRSDED
RGFKEDLNES
ATSPMRNRLD
DSNSRPGSQR
FVKSSRKEEE
TDSDSSSSKN
TTTRNNPIQY
TDKQQAELLR
KLDSIKDHLL
RGGGNNATVV
DQPPMGFHAH
HGPPPSYYNP
YPEPFPYGMY
PTASNQPHVP
AYRDPYGFPV
HRIPQNFYQG
PSHYPNQMPP
RPPYPQGQYV
DIGSDILESQ
LQDPRFFPGT
PSRYGDVPFS
PALHHGEKVG
PFSPHGGVHT
RWPSEIDSEM
GGAFARGYVQ
QAVSDTDSRR
CHPLAGGAPF
IACHSCFELL
YVPKKKLLGQ
ERQQKMQCGA
CSEVITFRVV
DKKLVFSSSA
LGETTNRVSV
EVEDRSSPIP
VVDDYPLNDE
EPRIHQETKI
VHAVSPSDHS
NDEDRSSISS
EPRKQVVKSV
RSRAQGAKVP
PPPPPEKSNL
LELFEYSNVN
RAAITYGMAQ
LGYYKQESYT
KQDSLKSESV
ATETDVSYNE
YYTNTEESED
SRISKASKEG
RRPRNRKQSS
EHSFAEVTNN
ISSNDQNNEQ
LEVWVNGYLI
PEDLVISAEK
QAGPVQAGKY
WYDYRAGFWG
VMGNPCLGII
PPFIEEFSRP
MPDNCGAGNT
SVFVNGRELH
ERDLELLSSR
GLPRGKNRSY
IIDIAGRVLD
GDSGEELKSL
GRLAPTVDKV
KHGFGMRVPR
SLVS
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.