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In the plant Arabidopsis thaliana, At1g01090.1 is a gene model for the locus At1g01090.[1]

Also known as PDH-E1 ALPHA, this gene model is classified by TAIR as "PDH-E1 ALPHA (PYRUVATE DEHYDROGENASE E1 ALPHA); pyruvate dehydrogenase (acetyl-transferring)". It is located on chr1, region 47705-49166 REVERSE.

Physiological functionEdit

At1g01090.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expressionEdit

At1g01090.1/Expression

Subcellular localizationEdit

At1g01090.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical propertiesEdit

The gene model At1g01090.1 has 428 amino-acid residues and a molecular weight Mw = 47.173 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 0.33, or 0.001 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotypeEdit

At1g01090.1/Knockout

Protein interactionsEdit

At1g01090.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structureEdit

The gene model At1g01090.1 has 1 recognized domains:

  • Domain_0089_0393 E1_dh domain (PF00676, IPR001017) Description: Dehydrogenase, E1 component;Biological Process: metabolism (GO:0008152), Molecular Function: oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor (GO:0016624)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequenceEdit

>At1g01090.1
MATAFAPTKL TATVPLHGSH ENRLLLPIRL APPSSFLGST RSLSLRRLNH SNATRRSPVV SVQEVVKEKQ STNNTSLLIT KEEGLELYED MILGRSFEDM CAQMYYRGKM FGFVHLYNGQ EAVSTGFIKL LTKSDSVVST YRDHVHALSK GVSARAVMSE LFGKVTGCCR GQGGSMHMFS KEHNMLGGFA FIGEGIPVAT GAAFSSKYRR EVLKQDCDDV TVAFFGDGTC NNGQFFECLN MAALYKLPII FVVENNLWAI GMSHLRATSD PEIWKKGPAF GMPGVHVDGM DVLKVREVAK EAVTRARRGE GPTLVECETY RFRGHSLADP DELRDAAEKA KYAARDPIAA LKKYLIENKL AKEAELKSIE KKIDELVEEA VEFADASPQP GRSQLLENVF ADPKGFGIGP DGRYRCEDPK FTEGTAQV
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-

Multiple sequence alignmentEdit

A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.

Protein structureEdit

A predicted structure for this protein is available here.

ReferencesEdit

  1. This is a test reference.

Further readingEdit

General database linksEdit

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