Wikia

In the plant Arabidopsis thaliana, At1g01010.1 is a gene model for the locus At1g01010.^[1]

Physiological functionEdit

At1g01010.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expressionEdit

At1g01010.1/Expression

Subcellular localizationEdit

At1g01010.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical propertiesEdit

At1g01010.1/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotypeEdit

At1g01010.1/Knockout

Protein interactionsEdit

At1g01010.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structureEdit

At1g01010.1/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequenceEdit

>At1g01010.1 MEDQVGFGFR PNDEELVGHY LRNKIEGNTS RDVEVAISEV NICSYDPWNL RFQSKYKSRD AMWYFFSRRE NNKGNRQSRT TVSGKWKLTG ESVEVKDQWG FCSEGFRGKI GHKRVLVFLD GRYPDKTKSD WVIHEFHYDL LPEHQRTYVI CRLEYKGDDA DILSAYAIDP TPAFVPNMTS SAGSVVNQSR QRNSGSYNTY SEYDSANHGQ QFNENSNIMQ QQPLQGSFNP LLEYDFANHG GQWLSDYIDL QQQVPYLAPY ENESEMIWKH VIEENFEFLV DERTSMQQHY SDHRPKKPVS GVLPDDSSDT ETGSMIFEDT SSSTDSVGSS DEPGHTRIDD IPSLNIIEPL HNYKAQEQPK QQSKEKVISS QKSECEWKMA EDSIKIPPST NTVKQSWIVL ENAQWNYLKN MIIGVLLFIS VISWIILVG ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-